摘要
目的 探讨静脉移植后内膜增生早期的发生机制。 方法 采用免疫组织化学、核酸原位杂交等方法 ,对大鼠静脉移植后 7天、14天、2 8天和 42天内膜增生中的 型、 型 [α1( )、α1( ) ]前胶原 m RNA及 型、 型胶原蛋白的表达进行定位、半定量观察。 结果 与组织修复相关的胶原合成于术后 7天出现 ,术后 14天消失。术后 14天起 ,α1( )、α1( )前胶原 m RNA开始大量在增生内膜的平滑肌细胞中表达 ,并于术后 2 8天达高峰 (P<0 .0 5 ) ,与相应胶原的表达呈同步增长。 结论 静脉移植后 7天 型、 型胶原即开始在内膜增生中的平滑肌细胞内转录及合成 ,且与平滑肌细胞的增殖无关。提示防治静脉移植后内膜增生至少应从术后 14天开始 ,并应包括抑制细胞增殖及胶原合成两个方面。
Objective\ To study the pathogenesis of intimal hyperplasia(IH) in early stage after vein to artery grafting.\ Methods\ With use of an established rat model of vein to artery transplantation, the expressions of α1(Ⅰ)、 α1(Ⅲ) mRNA and type Ⅰ、type Ⅲ collagen were located and semi quantified during the six weeks after operation.\ Results\ Collagen synthesis related to trauma healing lasted for 2 weeks after operation. After that, the expression of α1(Ⅰ)、α1(Ⅲ) mRNA increased gradually and reached the peak at the 4th week postoperative( P<0 05) . The expression of α1(Ⅰ)、α1(Ⅲ) mRNA and protein was increasing synchronously.\ Conclusion\ The transcription and synthesis of collagen were carried out 1 week postoperative, increased gradually and were not related to its proliferation. We suggest that the prevention of IH should include both anti proliferation and anti collagensynthesis and should be started at lease from the second week after operation.
出处
《中国胸心血管外科临床杂志》
CAS
2000年第2期99-102,共4页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
国家自然科学基金!( 3 95 0 0 14 0 )
关键词
静脉移植
内膜增生
胶原
平滑肌细胞
Vein to artery grafting Intimal hyperplasia Collagen Smooth muscle cells
