摘要
目的构建并鉴定能够分泌表达外源蛋白——屋尘螨抗原(Der p2)的基因重组耻垢分枝杆菌(rM.s)。方法采用电穿技术,将鉴定好的PCW-Der p2质粒转化到耻垢分枝杆菌(M.s),通过PCR鉴定目标基因在rM.s中的表达。结果经PCR鉴定,成功将PCW-Der p2质粒转化到M.s。结论成功构建了以分泌方式表达外源蛋白的Der p2-rM.s。
Objective To construct and identify the recombinant mycobacterium smegmatis（rM.s）which can secrete and express the exogenous antigen,namely dermatophagoides pteronyssinus antigen Der p2.Methods The identified PCW-Der p2 plasmid was transformed into M.s by the electroporation technology.And the expression of target gene in rM.s was identified by PCR technology.Results Through the identification by PCR,PCW-Der p2 plasmid was successfully transformed into M.s.Conclusion Der p2-rM.s,which can express foreign protein through the secretory way,is constructed successfully.
出处
《西南国防医药》
CAS
2012年第4期349-351,共3页
Medical Journal of National Defending Forces in Southwest China
基金
国家自然科学基金资助项目(30871114)
关键词
屋尘螨抗原
耻垢分枝杆菌
构建
鉴定
dermatophagoides pteronyssinus antigen
mycobacterium smegmatis
construction
identification
