摘要
我们首先用Alcian蓝染色的肥大细胞脱颗粒试验和用荧光测定组胺的方法观察了天花粉蛋白对肥大细胞脱颗粒和组胺释放的影响。实验结果表明:(1)在体外试验中,不论有无血清的存在,天花粉蛋白都不引起肥大细胞的脱粒和组胺释放。(2)天花粉蛋白的体内用药,能引起Balb/c小鼠炎症局部的肥大细胞脱颗粒(Alcian着色的未脱颗粒肥大细胞由对照组的3.4±0.12×10~4/ml减少至1.7±0.28×10~4/l;P<0.01)和组胺水平的明显增高(皮下气囊内的组胺水平由36.39±0.94ng/ml升至41.07±0.78ng/ml;P<0.01)。(3)腹膜腔内注射天花粉蛋白后,Wistatr大鼠腹膜腔内蛋白明显渗出,同时全血组胺水平也明显增高(对照组:0.947±0.076ng/ml,实验组:1.574±0.105ng/ml;p<0.01)。(4)上述天花粉蛋白诱导的腹腔渗出液具有明显的肥大细胞脱颗粒作用(脱粒指数:对照组为1.6±3.8%,实验组为24.9±2.1%;P<0.01)。(5)用眼镜蛇毒素完全耗竭小鼠的补体对天花粉蛋白引起的肥大细胞脱粒因子的产生无明显影响。从这些结果可见:天花粉蛋白的体内应用能通过激活某些可溶性成分的产生而引起肥大细胞的脱颗粒和组胺释放。但是,这一过程与天花粉蛋白激活补体的作用似无明显关系。
The effect of trichosanthin on mast cell degranulation and histamine release were observed with both Alcian blue staining method and fluorescent histamine determination method. It was showed that, ① in vitro trichosanthin showed no direct effect on the mast cell degranulation and histamine release even in the presence of fresh serum; ② in vivo administration on of trichosanthin in mice could cause significant mast cell degranulation and histamine release in inflammatory situ, as shown by the decreased number of Alcian blue stained nondegranulated mast cell in the peritoneal cavities; ③ intra peritoneal injection of trichosanthin in Wistar rats could significantly increase histamine level in the whole blood, while inducing prominent exudation of protein into the peritoneal cavities; ④ the supernatant of peritoneal exudate lavaged 12 hours after trichosanthin injection could cause mast cell degranulation. These results implicate that, although it showed no direct effect on mast cells, trichosanthin could cause mast cell to degranulate and release histamine via in vivo activation of some soluble mast cell degranulating factors. It was discovered in further study that the depletion of complement by cobra venom factor injection revealed no effect on the production of mast cell degranulation factor, suggesting that, in normal animals, the trichosanthininduced complement activation had no significant influence on the mast cell degranulation and histamine release.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1990年第5期272-276,共5页
Chinese Journal of Immunology
关键词
天花粉蛋白
肥大细胞
组胺释放
Trichosanthin
Mast cell
Degranulation
Histamine release
