摘要
目的探索流产模型孕鼠CD4+CD25+T细胞和CD4+CD25-T细胞快速高效的分选方法。方法采用免疫磁珠两步法分离流产模型孕鼠脾脏中T细胞,分离后的细胞经台盼蓝染色检测细胞存活率,经流式细胞仪检测分离的纯度。结果流产模型孕鼠脾脏细胞经过免疫磁珠细胞分选法2次分选得到CD4+/CD25+调节性T细胞纯度为(93.51.1)%,CD4+/CD25-T细胞纯度为(96.60.6)%。结论采用免疫磁珠细胞分选法能够快速高效的得到高纯度的CD4+CD25+T细胞和CD4+CD25-T细胞。
Objective To explore the method of separation of CD4＋CD25＋ T cells and CD4＋ CD25-T cells in abortion model pregnant rat fast and effectively.Methods The immune magnetic beads two-step separation is used to separate T cells of the spleen in abortion model rat,and the cell survival rate was tested by a cell to blue stain after separated,the flow cytometric tests the purity of separation.Results The purity of CD4＋CD25＋regulatory T cells was（93.51.1） % in abortion model pregnant rat by magnetic beads immune cell separation method for tow times,the purity of CD4 ＋CD25-T cells is（96.60.6） %.Conclusion The immune cells magnetic beads separation method of high speed and high efficiency can get high purity of CD4 ＋CD25＋ T cells and CD4 ＋CD25-T cells.
出处
《中国实用医药》
2012年第6期5-6,共2页
China Practical Medicine
基金
国家自然科学基金(项目编号：81170621)；国家人口和计划生育委员会立项项目(项目编号：C-73)；山西省科学技术发展计划项目(项目编号：2008031lO72)
关键词
流产模型
调节性T细胞
免疫磁珠细胞分选
流式细胞仪检测
Abortion model
Regulatory T cells
Immune cell sorting magnetic beads
Flow cytometric detection
