摘要
本文报道一种化学法制备生物素标记核酸探针的方法。先在亚硫酸氢钠催化下用乙二胺(或已二胺)对核酸的胞嘧啶残基进行化学修饰,然后与生物素-N-羟基琥珀酰亚胺酯反应将生物素标记于核酸上。用此法制备了λDNA探针并进行了打点、Southern杂交检测。结果表明,此法具有敏感、特异、重复性好、经济等特点,可望得到推广应用并部分取代同位素标记的探针。
A chemical method to prepare biotin-labeled nucleic acid probe for nonisotopic hybridization is introduced. The method involves the bisulfite-catalyzed transamination reaction of cytosine residues in nucleic acids with ethylenediamine or hexanediamine. Primary amino groups on the cytosine derivatives are then reacted with biotiny-N-hydroxysuccinimide ester. Results of dot blot and Southern blot carried out with biotinlabeled λ DNA probe show that such nonisotopic probe method is sensitive, specific, repeatable and unexpensive.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1990年第4期244-247,共4页
Chinese Journal of Immunology
关键词
生物素
核酸探针
化学标记
Biotin-Labeled Nucleic Acid Probe
Chemical Labeling
Hybridization
