摘要
于１９９７年１０月－１９９８年１月，从胶州湾贝类养殖场购买栉孔扇贝，分别采用邻苯三酚自氧化法和过氧化氢法测定了其血细胞和血清中的超氧化物歧化酶（ＳＯＤ）和过氧化氢酶（ＣＡＴ）的活力，并对ＳＯＤ类型的鉴别区分、ＳＯＤ和ＣＡＴ的热稳定性以及温度对ＳＯＤ和ＣＡＴ活力的影响进行研究。结果表明，血细胞中ＳＯＤ和 ＣＡＴ活力分别为３１２．７Ｕ和 １２４．４Ｕ，血清中ＳＯＤ和ＣＡＴ活力分别为１０２．３Ｕ和７３．８Ｕ；冻存１０ｄ后，血清中ＳＯＤ的活力丧失２４．６％，而ＣＡＴ的活力丧失４０．８％；血细胞中ＣｕＺｎ－ＳＯＤ和Ｍｎ－ＳＯＤ的活力分别为１９３．４Ｕ和８２．３Ｕ，血清中仅存在ＣｕＺｎ－ＳＯＤ活性；血清中ＳＯＤ的热稳定性很高，在８０℃下保温３０ｍｉｎ后，酶活力仍很高，而ＣＡＴ的热稳定性较低；血清中的ＳＯＤ和ＣＡＴ的最适温度分别为５０℃和４５℃。
The activities and properties of superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.1 1.1.6) in the haemocytes and serum of Chlamys farreri were studied from October 1997 to January 1998. The activities of SOD were measured with the method of autooxygenation of pyrogallol. The activities CAT were measured by the decrease in absorbance due to H_2O_2 consumption. The distinction of CuZn and Mn form of SOD, heat stabilities of SOD and CAT, effects of temperature on the activities of SOD and CAT were also studied. There are activities of SOD and CAT in both haemocytes and serum. SOD and CAT activities in haemocytes are 312.7U and 124.4U; and are 102.3U and 73.8U in serum.. 24.6% and 40.8% of SOD and CAT activities in serum are reduced after they were iced for 10 days. CuZn-SOD and Mn-SOD activities in haemocytes are 70.3% and 29.7% of the total SOD activities. CuZn-SOD is present but Mn-SOD is not detectable in serum. The heat stability of SOD is very high. SOD is highly active after the serum was beated in 80℃ for 30min. The heat stability of CAT is low. The optimum temperature for SOD and CAT in serum respectively appear about 50℃ and 45℃.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2000年第3期259-265,共7页
Oceanologia Et Limnologia Sinica
基金
国家攀登计划B资助项目!PD-B6-6-3
关键词
栉孔扇贝
超氧化物歧化酶
血淋巴
过氧化氢酶
Chlanys farreri Superoxide dismutase (SOD) Catalase (CAT) Haemolymph Haemocyte Serum
