摘要
目的:观察NASH大鼠肝组织过氧化物酶体增殖物激活受体(PPARα)mRNA表达变化及加减王氏连朴饮对其影响。方法:采用高脂饮食复制大鼠NASH模型。分为正常对照组、空白模型组、加减王氏连朴饮组、东宝肝泰(复方蛋氨酸胆碱片)对照组。测定各组血清甘油三酯(TG)、总胆固醇(TCH)、低密度脂蛋白(LDL)和丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平;原位杂交技术检测肝组织PPARαmRNA的表达。结果:与正常对照组相比,空白模型组大鼠肝组织PPARαmRNA表达明显减弱(P<0.01);与空白模型组相比,加减王氏连朴饮组和东宝肝泰对照组大鼠肝组织PPARαmRNA表达明显增强(P<0.05),加减王氏连朴饮组和东宝肝泰对照组之间没有显著性差异(P>0.05)。结论:PPARαmRNA表达减弱引起脂质代谢失衡参与NASH的发病。清热化湿方通过激活PPARαmRNA表达来调节脂质代谢,发挥防治NASH的作用。
Objectives ：To study the effect of heatclearing and damp -draining preparation on mRNA expression of peroxisome Prolifreceptor α （ PPARα ） in hepatic tissue of rats with nonalcoholic steatohepatitis. Methods：The NASH models of rats were made by high fat diet. The rats were randomly divided into normal control, mode, formula of heat - clearing and damp - draining preparation and Compound methionine and cho- line control group. The content of scram triglyceride（TG）, total cholesterol （TCH）, low density lipoprotein （ LDL）, aminotransferase （ALT） and aspartate aminotransferase （AST） were detected with Olympus Au560 en- tire automated biochemical analysis instrument, mRNA expression of PPARαin hepatic tissue of rats was detected by using in situ hybridization. Results： Compared with the normal control group, the PPARa mRNA expression of hepatic tissue of the blank model group was degraded obviously（P 〈0.01 ）. Compared with the blank model group, Formula of heat - clearing and damp - draining preparation and Compound Methionine and Choline Tablet could reinforce the PPARa mRNA expression of hepatic tissue （ P 〈 0. 05 ）, the difference of the effect between Formula of heat - clearing and damp - draining preparation group and Compound Methio- nine and Choline Tablet control group wasn＇ t significant in statistics （ P 〉 0.05 ）. Conclusion ： The PPARα mRNA expression of hepatic tissue degraded are nosogenesis of NASH. Formula of heat - clearing and damp - draining preparation can increase the PPARαmRNA expression in hepatic tissue.
出处
《中医药信息》
2012年第2期101-104,共4页
Information on Traditional Chinese Medicine
基金
湖北省卫生厅中西结合课题(鄂卫发【2007】56号)
