摘要
目的:分析急性阑尾炎中分离的大肠埃希菌产超广谱β-内酰胺酶(extended spectrumβ-lactamases,ESBLs)的发生率,并进行耐药分析。方法:对沈阳医学院沈洲医院2010年1月至2011年12月收治的73例急性阑尾炎标本进行细菌培养及菌株分离,用纸片扩散法对分离的菌株进行13种抗生素的敏感性试验,用双纸片做ESBLs的确证试验。结果:73例急性阑尾炎标本可分离出菌株50株,其中大肠埃希菌41株(82.00%),其中ESBLs阳性15株(36.59%),ESBLs阴性26株(63.41%),ESBLs阳性菌株对头孢类存在广泛耐药,对β-内酰胺类抗生素和β-内酰胺酶抑制剂的混合制剂仍有较高的敏感性,对碳青霉烯类抗菌药物100%敏感。结论:ESBLs阳性菌对常用抗生素耐药严重,各单位和地区对产ES-BLs菌的耐药要严密监测,根据实验室药敏结果,及当地细菌的耐药状况综合考虑,以指导临床合理和应用抗生素。
Objective：To analyze the incidence and drug resistance of extended spectrum β-lactamases（ESBLs） produced by E.coli separated from acute appendicitis for guidance of suitable clinical mediation.Methods：The bacteria were isolated and cultured from specimens of 73 cases with acute appendicitis at Shenzhou hospital affiliated to Shenyang Medical College,from January 2010 to December 2011.Drug sensitivity test of ESBLs using 13 kinds of antibiotics was done with the disk diffusion method,and the confirmed test was done with the double disk test.Results：Fifty strains were separated from specimens of 73 cases with acute appendicitis.Among them,41 strains were Escherichia coli（82.00%）,with 15 strains were ESBLs positive（36.59%） and 26 strains were ESBLs negative（63.41%）.The ESBLs strains were extensively drug-resistant to Cephalosporins and Cephamycins,but had a high sensitivity to the mixed beta-lactam antibiotics and β-lactam inhibitors,and were entirely sensitive to carbapenems antimicrobial agents.Conclusions：ESBLs were seriously resistant to commonly used antibiotics.Drug resistance of ESBLs should be closely monitored to guide rational use of antibiotics according to the results of drug susceptibility and local bacterial resistance situation.
出处
《沈阳医学院学报》
2012年第1期19-20,25,共3页
Journal of Shenyang Medical College
关键词
阑尾炎
大肠埃希菌
Β-内酰胺酶
药敏分析
appendicitis
E.coli
spectrum β-lactamases（ESBLs）
analysis of drug
