摘要
目的研究前列地尔联合雷米普利对阿霉素肾病大鼠肾脏NF-κB表达的影响,探索其作用机制。方法正常六周龄雄性wistar大鼠40只随机分为四组。模型组10只,单用前列地尔组10只,单用雷米普利组10只,前列地尔联合雷米普利组10只,相应药物干预后分别于4周、8周末进行24h尿蛋白定量、ALB、Scr、Cys-c检测,免疫组化方法测定肾脏NF-κB的表达。结果与模型组和单用药物治疗组相比,4W、8W末,前列地尔联合雷米普利组大鼠24小时尿蛋白定量,血浆Cys-c,肾脏组织NF-κB的表达均显著降低(P<0.01)。结论前列地尔联合雷米普利可降低阿霉素肾病大鼠肾脏NF-κB表达,减少蛋白尿,延缓肾小球硬化。
Objective To investigate the effects of Alprostadil combined with Ramipril on expression of NF -κBin rats with Adriamysin Nephropathy （ADR）. Methods 40 Wistar rats were randomly divided into ADR group, ramipril treated group, Alprostadil treated group and ADR group, ramipril treated group, Alprostadil treated group After administration for 4 weeks and 8 weeks,24h urinary protein,ALB,Scr,Cys-C were measured. The expression levels of NF -κB, was examined by immunohistochemistry. Results Compared with ADR group, ramipril treated group and Alprostadil treated group,the levels of 24hUP,Cys-C were significantly decreased in Alprostadil combined with Ramipril treated group （P0.05）. In immunohisto chemistry, the expression of NF-κB in Alprostadil treated group, ramipril treated group, Alprostadil treated group and Alprostadil combined with Ramipril treated group were significantly lower especially in Alprostadil combined with Ramipril treated group. Conclusion Alprostadil combined with Ramipril can reduce proteinuria through deceasing the expression of NF-κBin adriamycin nephropathy.
出处
《当代医学》
2012年第11期29-30,共2页
Contemporary Medicine
