摘要
为确诊某规模化奶牛场奶牛爆发恶性水肿死亡病例,采取病变组织触片染色,分离培养,分离菌形态观察,生化鉴定、实验动物接种、16SrRNA同源性序列分析鉴定。结果显示:病死牛肝触片染色菌体形态、分离株培养及菌体形态、生化反应均符合腐败梭菌特征;分离株PCR产物测序后与GenBank已发表的参考基因相应片段同源性比较,同源性为95.43%;分离株培养物经肌肉与腹腔分别接种豚鼠及小鼠后均引起死亡,肝触片染色镜检,其菌体形态与奶牛病例相同。结果表明,该奶牛场奶牛死亡系由腐败梭菌感染引起的恶性水肿所致。
In order to diagnose the large-scale cow death cases caused by malignant edema at a diary farm,the following steps are include：the pathological changes of the tissues were stained,isolated and cultured,then the Morphological observation of isolated bacteria,identification of biochemistry and pathogenicity test of animal physiology and 16S rRNA homologous sequence identification were conducted.The results show that the liver tissue smear from the dead cow,the cultivation of the isolates,bacterial morphology and biochemical reactions are all in accordance with clostridium speticum.Based on PCR products sequencing,compared with corresponding fragment of the published reference gene,the homology identification was 95.43%.The muscular and intraperitoneal injection of the isolated culture caused death of guinea pigs and mice,The liver tissue smear was prepared,and the morphology was the same as the cow death cases.The results proved that the cows of this dairy farm died from the malignant edema caused by the clostridium septicum.
出处
《石河子大学学报（自然科学版）》
CAS
2012年第1期47-50,共4页
Journal of Shihezi University(Natural Science)
关键词
恶性水肿
腐败梭菌
分离鉴定
PCR
malignant edema
Clostridium septicum
isolation and identification
PCR
