摘要
以芦丁为对照品，NaNO2-Al（NO3）3-NaOH为显色系统，用紫外分光光度法，在498nm波长测定白千层枝和叶中总黄酮的含量。结果显示，其回归方程为A=0.01022C＋0.0165，r=0.9995，吸光度在0-100mg／L范围内呈良好线性关系。测定结果表明，白千层枝和叶中总黄酮的质量分数分别为0.69％和0.63％。
A The total flavonoids content in the stems and leaves of Melaleuca leucadendron Linn was determined by spectrophotometry with rutin standard and NaNO2-Al（NO3）3-NaOH system. The regression equation was A=0.01022C＋ 0.0165 （r=0.9995）.The results showed that the mass fractions of total flavonoid in the stems and leaves were 0.69% and 0.63% respectively. The method was stable, accurate, reproducible, highly sensitive and thus was a realistic and practi cal approach for determining flavonoid content of Melaleuca leucadendron linn.
出处
《海南师范大学学报（自然科学版）》
CAS
2012年第1期63-65,共3页
Journal of Hainan Normal University（Natural Science）
基金
海南师范大学国家大学生创新实验项目(101165804)
关键词
白千层
总黄酮
紫外分光光度法
Melaleuca leucadendron Linn.
flavonoid
ultraviolet spectrophotometric method
