摘要
为了研究CAPN10基因的性质和功能,使用NCBI等网站提供的一系列在线工具和软件,对其基因序列及其编码蛋白的结构和特性进行生物信息学分析。结果表明,野猪CAPN10基因CDS全长2 004 bp,编码667个氨基酸,同源性比对发现CAPN10保守性较低,对选择编码氨基酸的密码子具有偏好性;进一步的蛋白特性分析表明,野猪CAPN10是一种等电点为8.24的不稳定水溶性蛋白,无信号肽序列和跨膜结构,具有磷酸化等功能化位点及钙蛋白酶类催化基序结构。
In order to study the nature and function of gene CAPN10,bioinformatics analysis of its gene sequence and the structure and properties of its encoded protein was preformed by a series of online tools and software offered by NCBI and others sites.The results showed that the entire length of the Sus scrofa Ussuricus CAPN10 gene CDS sequence,encoding 667 amino acids,was 2 004 bp,homology comparison indicated that the conservation of CAPN10 was lower and the CAPN10 gene preferred a codon encoding selectively amino acid.Further analysis of the protein characteristics suggested that Sus scrofa Ussuricus CAPN10 whose isoelectric point was 8.24,was an unstable and water-soluble protein,with many functional sites such as phosphorylation sites and calcium protease catalytic motif structure.No signal peptide sequence and transmembrane structure were detected in this protein.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2012年第3期24-28,共5页
Journal of Northeast Agricultural University
基金
黑龙江省教育厅科学技术研究项目(11551033)
中国博士后科学基金项目(200902426)
