摘要
将合浦珠母贝三倍体的卵与二倍体的精子授精 ,用 0 .5 μg/mL细胞松弛素B抑制受精卵第一极体的释放诱导四倍体。研究了处理起始时间及持续时间对胚胎孵化率和四倍体诱导率的影响及幼虫的生长及存活。实验结果表明 :持续时间与胚胎孵化率呈负相关 ,而与四倍体诱导率呈正相关 ,持续时间一般为1 5～ 1 8min ,处理起始时间一般在第一极体出现前 3～ 5min。在胚胎期 ,四倍体诱导率平均为 2 0 %。在幼虫培养阶段 ,幼虫死亡严重 ,收苗率极低 ,平均只有 0 .0 2 %。处理组与未处理组的生长差异不明显 (p >0 .0 5 ) ,但两组幼虫生长明显慢于二倍体组 (p <0 .0 5 )。
Tetraploidy was induced by inhibiting the first polar body in eggs from triploid Pinctada martensii (Dunker) fertilized with haploid sperms with 0.5μg/mL cytochalasin B (CB) treatment.The effects of treatment duration and timing of treatment after fertilization on embryonic hatching rate and the percentage of tetraploid were examined, the growth and survival of larvae were compared between treated group, untreated group and diploid control. The results indicated that the hatching rate was inversely related to treatment duration time, but the percentage of tetraploid was positively related to treatment duration time. Two optimal moments for treatment application were determined. Optimal treatment duration time was 15 18min, optimal timing of treatment was 3 5min before the release of polar body 1.The percentage of tetraploidy embryos was 20% averagely, the percent harvest of spat was only 0.02% due to a heavy mortality of larvae. There was no difference in growth of larvae between treated group and untreated group(p >0.05), but the growth of larvae in both groups was slower than that of diploid control and the difference was significant (p<0.05).
出处
《水产学报》
CAS
CSCD
北大核心
2000年第1期22-27,共6页
Journal of Fisheries of China
基金
国家863资助!(合浦珠母贝多倍体育种和养殖研究 )
863 -819-0 1-0 3号
关键词
合浦珠母贝
三倍体
诱导
四倍体
细胞松弛素B
Pinctada martensii, triploid, inducement,tetraploid, cytochalasin B
