摘要
目的比较涂片镜检查找革兰阳性粗大杆菌与实时荧光定量PCR这两种方法对快速诊断产气荚膜梭菌的意义。方法选取疑似气性坏疽且伤口分泌物涂片镜检发现革兰阳性粗大杆菌的患者16例,取其伤口分泌物进行实时荧光定量PCR及细菌培养。结果 16例涂片镜检查到革兰阳性粗大杆菌的患者中,有6例实时荧光定量PCR为阳性并培养出产气荚膜梭菌。结论与传统的涂片方法相比,实时荧光定量PCR方法能更准确,更灵敏地诊断产气荚膜梭菌。
Objective To compare using smear examination in searching gram positive bacillus method with real - time quantitative PCR method in rapid diagnosis of clostridium perfringens. Methods 16 patients with suspected gaseous gangrene who were also found gram positive bacillus by the examination of wound secretion smear were assigned to receive real - time quan-titative PCR and be performed wound smear bacteriological culture. Results 6 out of the 16 patients were confirmed positive by real -time quantitative PCR and at the same time their wound smear bacteriorlogical culture was also found clostridium perfrin- gens. Conclusion Real - time quantitative PCR is more accurate and sensitive for rapid diagnosis of clostridium perfringenscompared with the traditional method of smear examination.
出处
《中国全科医学》
CAS
CSCD
北大核心
2012年第8期913-914,共2页
Chinese General Practice
基金
全军医学科学技术研究"十一五"重大专项(08Z009)
