摘要
以微乳液相色谱法分离测定普伐他汀钠、阿托伐他汀钙、辛伐他汀和洛伐他汀.考察了微乳液组成表面活性剂质量分数、油相质量分数、助表面活性剂质量分数以及pH值等对他汀类药物分离测定的影响,确定最佳的色谱条件.实验结果表明:等梯度微乳液相色谱具有较强的同时快速分离疏水性差异较大的混合物的能力.该方法具有操作简便、快速,准确性和重现性好等特点.对四种制剂供试品多次测定,普伐他汀钠、阿托伐他汀钙、辛伐他汀和洛伐他汀的相对标准偏差(RSD)分别为0.39%,0.45%,0.053%和0.25%.加入回收率分别为97.8%～101.2%,101.5%～102.7%,97.7%～101.9%和98.7%～101.9%.
Pravastatin sodium,atorvastatin calcium,lovastatin and simvastatin were determined by microemulsion liquid chromatography.The effect of concentration of surfactant,cosurfactant and lipophilic organic solvent and pH value on determination and separation efficiencies of statins had been investigated to get the best chromatography conditions.The experimental results showed that isogradic microemulsion liquid chromatography posses better ability in rapid separation of statins mixture which has a very different hydrophobicity.It is a simple,fast,accurate and precision method for the determination of pravastatin sodium,atorvastatin calcium,lovastatin and simvastatin.The repeated tests of four drugs showed that RSD for pravastatin sodium,atorvastatin calcium,lovastatin and simvastatin were 97.8%~101.2%,101.5%~102.7%,97.7%~101.9% and 98.7%~101.9%,respectively.
出处
《浙江工业大学学报》
CAS
2012年第2期119-123,共5页
Journal of Zhejiang University of Technology
关键词
微乳液相色谱
普伐他汀钠
阿托伐他汀钙
辛伐他汀
洛伐他汀
含量测定
microemulsion liquid chromatography
pravastatin sodium
atorvastatin calcium
lovastatin
simvastatin
content determination
