摘要
目的调查我院2010年临床分离病原菌对抗菌药物的耐药性。方法采用纸片扩散法进行抗菌药物敏感实验,根据CLSI2010采用WHONET5.4软件进行数据分析。结果临床分类的1993株病原菌中,革兰阳性菌占21.2%,革兰阴性菌占78.8%;耐甲氧西林金黄色葡萄球菌和凝固酶阴性葡萄球菌株检出率分别为56.7%和91.9%;发现2株对万古霉素和替考拉宁耐药的屎肠球菌,表型及基因型检测为vanA型耐药;未发现对万古霉素或替考拉宁中介或耐药的葡萄球菌和粪肠球菌菌株。大肠埃希菌、肺炎克雷伯菌ESBLs检出率分别为61.7%、38.8%,大肠埃希菌ESBLs的菌株高于2008年(51.7%)和2009年(57.2%)监测比例。鲍曼不动杆菌对碳青霉烯类抗菌药物的耐药率在65%以上。结论对当地医院进行定期的细菌耐药监测,发现细菌的耐药变化趋势,对规范临床合理使用抗菌药物具有重要意义。
Objective To investigate the antimicrobial resistance of clinical isolates from the First Affiliated Hospital of Chongqing Medical University in 2010. Methods Disk diffusion method was used to determine the antimicrobial susceptibility. And the WHONET 5.4 software was used to analyze the data based on the CLSI2010. Results Of 1993 clinical isolates, gram-positive organisms accounted for 21.2%, and Gram-negative organisms 78.8%. The detection rates of methicillin-resistant Staphylococcus aureus （MRSA）and coagulase-negative staphylococci （CNS） were 56.7% and 91.9%, respectively. Two strains of Enterococcusfaecium were found resistant to vancomycin and teicoplanin. They were confirmed as vanA by phenotypic and genetic analysis. Extendedspectrum β-lactamases （ESBLs）-producing Escherichia coli and Klebsiella sp. were accounted for 61.7% and 38.8%, respectively. The frequency of ESBLs -producing E. coli was higher than that obtained in 2008 and 2009. The carbapenem resistance rate in Acinetobacter sp. was more than 65%, respectively. Conclusion Periodically bacterial resistance surveillance is of great importance for clinically rational usage of antimicrobials.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2012年第4期291-297,共7页
Chinese Journal of Antibiotics
基金
中国Chinet细菌耐药监测组部分资助
关键词
细菌
耐药
抗菌药物敏感性
Bacteria
Resistance
Antimicrobial susceptibility
