摘要
目的探讨茵陈注射液对梗阻性黄疸幼鼠肝损伤的保护作用。方法 Wistar幼龄大鼠80只随机分为4组:假手术组、梗阻性黄疸模型组、茵陈多糖高(0.8mg/kg)、低剂量(0.4mg/kg)干预组;后3组采用胆总管结扎法制作梗阻性黄疸模型,于术后当天分别给药,并于术后1、2周分别测定4组幼鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、间接胆红素(IBIL)、直接胆红素(DBIL)及肝组织匀浆中丙二醛(MDA)、谷胱甘肽(GSH)、过氧化物岐化酶(SOD)等。结果茵陈多糖高、低剂量干预组术后1、2周幼鼠血清ALT、AST活性及TBIL、IBIL、DBIL含量明显下降;肝组织匀浆中MDA含量明显降低,GSH含量及SOD活性明显升高,与梗阻性黄疸模型组比较,差异均有统计学意义(P<0.05)。结论茵陈多糖对梗阻性黄疸幼鼠肝损伤具有一定的保护作用。
Objective To investigate the protective effects of Capillary artemisia polysaccharide on hepatic injury induced by obstructive jaundice in immature rats. Methods Eighty immature Wistar rats were randomly divided into four groups, sham-operation group, obstructive jaundice model group, high-dose （0.8mg/kg） and low-dose （0.4mg/kg） polysaccharide intervention groups. Except for the sham-operation group, the obstructive jaundice models were made by double-ligating choledochus in the other three groups, and the rats were administered respectively. The levels of serum ALT, AST, TBIL, IBIL, DBIL and the levels of MDA, GSH, SOD in hepatic tissue homogenate were detected at 1 week and 2 weeks after the operation for the four groups. Results The activities of ALT, AST and the contents of TBIL, IBIL, DBIL in high-dose and low-dose polysaccharide intervention groups at 1 week and 2 weeks after the operation were significantly decreased, and the content of MDA in hepatic tissue homogenate was obviously reduced, however, the levels of GSH and activity of SOD were significantly increased, and there were significant differences in these indexes between the intervention groups and obstructive jaundice model group （ P 〈 0.05 ）. Conclusion Capillary artemisia polysaccharide has certain protective effects on hepatic injury induced by obstructive jaundice in immature rats.
出处
《河北医药》
CAS
2012年第8期1128-1130,共3页
Hebei Medical Journal
关键词
茵陈多糖
黄疸
阻塞性
幼鼠
肝损伤
Capillary artemisia polysaccharide
jaundice, obstructive
immature rats
hepatic injury
