摘要
目的探讨甲状腺素对大鼠肝脏缺血再灌注后血红素加氧酶-1(HO-1)表达的影响及其对肝脏的保护作用与机制。方法建立70%大鼠肝脏缺血再灌注损伤模型(缺血1h,再灌注6h),将30只雄性Sprague-Dawley大鼠随机分为假手术组、对照组、处理组三组,每组10只。处理方法为于缺血48h前0.1mg/kg的T3腹腔注射。测定再灌注末血清ALT、AST和肝脏组织丙二醛(MDA)、超氧化物歧化酶(SOD)的变化,HE染色光镜下肝组织学观察。应用Westernblot及RT-PCR检测肝脏HO-1的表达。结果与假手术组相比,对照组细胞上清液中ALT、AST和MDA含量明显较高(P<0.05),SOD明显较低(P<0.05);肝细胞坏死严重,排列紊乱。处理组的ALT、AST和MDA显著低于对照组(P<0.05),SOD明显高于对照组(P<0.05);肝细胞坏死减轻,排列相对整齐。假手术组有少量HO-1表达,处理组HO-1表达高于对照组,差异有统计学意义(P<0.05)。结论甲状腺素能上调HO-1的表达以减少肝细胞坏死,减轻大鼠肝脏缺血再灌注损伤,从而对肝脏有保护作用。
Objective To explore the effect of thyroid hormone on heme oxygenase-1（HO-1）expression after liver ischemia reperfusion injury in rat.Methods An ischemia-reperfusion（I-R）process of 1 h ischemia and then 6 h reperfusion was established in male Sprague-Dawley rat.The 30 rats were randomly divided into three groups;sham-operated,control,treatment.Treatment of 0.1 mg/kg intraperitoneal injection of T3 at 48 h before ischemic.The levels of ALT,AST in serum and malondialdehyde（MDA）and superoxide dismutase（SOD）in liver tissue were detected at reperfusion for 6 hours.The expression of HO-1 were examined by Western blot and RT-PCR.Results Compared to the control group,the expression of the HO-1mRNA and protein was higher in the treatment group.The level of ALT,AST in serum samples and MDA in liver of the treatment group were significantly lower than that of the control group（P0.05）and the level of SOD was higher（P0.05）.Histology showed an severe necrosis in the control group.The necrosis was ameliorated in the treatment group.Conclusions These results indicate that thyroid hormone up-regulate the expression of HO-1 and decrease the liver ischemic reperfusion injury.
出处
《中华临床医师杂志（电子版）》
CAS
2012年第1期17-20,共4页
Chinese Journal of Clinicians(Electronic Edition)
