摘要
目的:研究云芝糖肽(PSP)对人外周血单个核细胞(PBMC)中TLR5信号通路的调控作用,探索PSP对Toll样通路的免疫调节机制。方法:体外分离、培养人PBMC,分为对照组和PSP处理组,采用实时荧光定量PCR(Q-PCR)技术对PSP处理人PBMC前后,PBMC中的TLR5信号通路中相关基因的表达差异情况进行定量分析。结果:PSP处理的实验组与对照组相比较,TLR5信号通路中的TLR5、IRAK4、TRAF6、IRF5和NF-κB基因的表达水平均显著增高(P<0.05),但AP-1基因的表达降低(P<0.05)。结论:PSP对PBMC中TLR通路的调控可能主要通过NF-κB和IRF5调控终端效应因子来发挥免疫调节作用,这些为进一步探讨PSP的免疫调节作用机理提供了实验依据。
Objective：To detect the effects of polysaccharopeptide（PSP） on TLR5 signaling pathway in human PBMC,and explore the immunoregulatory mechanism of PSP.Methods：The human PBMC was isolated and cultured in vitro and then divided into control group and PSP group.Real time fluorescent quantitative PCR（Q-PCR）was used to quantitatively analyze the expression of the genes of TLR5 signaling pathway in different groups.Results：The expression levels of TLR5,IRAK4,TRAF6,NF-κB and IRF5 in the PSP group were significantly higher than these in the control group except the AP-1（P0.05）.Conclusion：PSP could play a role of regulation with the expressions of NF-κB and IRF5 mRNA in the TLR on the PBMC,and could supply evidences for further exploring the immunoregulatory mechanism of PSP.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第3期217-220,共4页
Chinese Journal of Immunology
基金
重庆市科委课题项目(CSCT 2009BB5399)
重庆市教委课题项目(KJ110303)
重庆市卫生局中医药科技项目(2010-2-147)资助
关键词
云芝糖肽
TLR5
免疫调节
实时荧光定量PCR
Polysaccharopeptide
TLR5
Immunoregulation
Real time fluorescent quantitative PCR
