摘要
载体构建是利用农杆菌介导建立真菌遗传转化体系的基础。以双元载体pPZP100为骨架,通过限制性内切酶酶切、连接以及去磷酸化将标记基因NptⅡ及StNPS6基因侧翼序列导入目标载体pPZP100中,构建玉米大斑病菌非核糖体肽合成酶6基因敲除载体,并采用冻融法将pPZP100NptⅡNPS6转入农杆菌菌株AGL-1。
Vector construction was on the basis of establish the genetic transformation system of fungi with Agrobacterium. In this study, Npt Ⅱand StNPS6 flanking sequence were linked into pPZP100 by restriction enzymes, remove phosphorylation and connection. Deletion vector of nonribosomal peptide synthetases （NPS6） of Setosphaeria turcica had been constructed.pPZP100Npt Ⅱ NPS6 was transformed AGL-1 by method of freeze-thaw.
出处
《玉米科学》
CAS
CSCD
北大核心
2012年第2期59-63,共5页
Journal of Maize Sciences
基金
教育部科学技术研究重点项目"非核糖体多肽合成酶NPS6基因调控玉米大斑病菌致病机制研究"(211038)
关键词
玉米
大斑病菌
非核糖体肽合成酶6
敲除载体
Maize
Setosphaeria turcica
Nonribosomal peptide synthetases 6
Deletion vector
