摘要
目的研究不同精子活力的人精子中精子特异性阳离子通道蛋白1(CatSper1)mRNA的表达差异,以及其与精子活力相关指标的相关性。方法分别收集少、弱精症患者精液标本和健康人对照精液标本各25份,用Trizol试剂提取精子中的总RNA,逆转录聚合酶链反应(RT-PCR)检测CatSper1 mRNA表达改变。结果少、弱精症组CatSper1 mRNA表达水平与健康人对照组相比,差异具有统计学意义[(0.54±0.01)vs(1.13±0.05),P<0.01]。CatSper1 mRNA表达与精子活率以及a+b级精子百分率之间呈显著正相关。结论人精子CatSper1 mRNA表达与人精子活力呈正相关。
Objective To study the differentially expressed of CatSper1 mRNA in human mature spermatozoa with different motility,and correlation between sperm-related indicators and the expression of CatSper1 mRNA.Methods Semen specimens of 25 oligo-atheno-spermia patients and 25 normal subjects were collected.Total RNA of human of mature spermatozoa was isolated by Trizol reagent.The expression of CatSper1 mRNA was detected by reverse transcription-polymerase chain reaction（RT-PCR）.Results Comparing the expression of CatSper1 mRNA between oligo-atheno-spermia patients and normal controls,a difference with statistical significance was found [（0.54±0.01） vs（1.13±0.05）,P0.01].The results indicated significant positive correlation between CatSper1 mRNA expression and proportion of sperm with motility of grade（a＋b）.Conclusion Expression of CatSper1 mRNA may positively correlate with human sperm motility.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2012年第3期222-223,共2页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金(81070480)
全军"十一五"医药卫生科研基金(07Z031)
