摘要
以A40926 B产生菌野野村放线菌SIPI-D-30为出发菌株,通过UV诱变及乙酸钠和甘氨酸抗性平板筛选,获得A40926 B产量提高的突变株SIPI-U-157。经优化,确定摇瓶发酵培养基(g/L)为:葡萄糖5.0、麦芽糊精30.0、可溶性淀粉10.0、玉米淀粉30.0、大豆蛋白胨5.0、肉蛋白胨10.0、酵母浸粉5.0、冷榨黄豆饼粉10.0、棉籽饼粉5.0、L-缬氨酸1.0,发酵培养7 d,突变株的A40926 B发酵单位为893 mg/L。该突变株在5 L发酵罐中培养180 h,A40926 B的产量最高为583 mg/L。
A medium for Nonomuraea sp. SIPI-D-30 fermentation was developed and the productivity of glycopeptide A40926 B was improved by strain breeding. After SIPI-D-30 was treated with UV and screened in the resistant plate of sodium acetate and glycin, a mutant SIPI-U-157 with higher production ofA40926 B was obtained. The effects of carbon and nitrogen sources on the antibiotic production in shake-flask culture were investigated. The optimized medium compositions （g/L） were glucose 5.0, maltodextrin 30.0, soluble starch 10.0, corn starch 30.0, soy peptone 5.0, meat peptone 10.0, yeast extract 5.0, soybean meal 10.0, cotton seed meal 5.0 and L-valine 1.0, in which the production of A40926 B by strain SIPI-U-157 reached 893 mg/L after 7 days＇ cultivation. In addition, a fed-batch culture of SIPI-U-157 in a 5 L fermentator also conducted. The yield ofA40926 B was 583 mg/L at 180 h.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2012年第4期256-259,共4页
Chinese Journal of Pharmaceuticals
基金
微生物药物技术创新与新药创制产学研联盟(2010ZX09401-403)
