摘要
目的:观察病毒性心肌炎(VMC)小鼠心肌骨桥蛋白(OPN)及Ⅰ型胶原(Col Ⅰ)的表达,探讨OPN在VMC发病中的作用机制。方法:以柯萨奇病毒B3(myocarditic coxsackievirus B3,CVB3)感染BALB/C小鼠建立VMC模型,取心肌组织行HE和Masson染色,并计算病理积分及心肌胶原纤维容积分数(CVF);RT-PCR检测Col I含量;RT-PCR及ELISA法检测OPN表达。结果:VMC组第7天心肌细胞大量坏死,炎性改变明显,随后炎性病灶逐渐吸收,第28天病灶周围炎细胞几近消失,纤维化明显;VMC组各时间点心肌病理积分、OPN mRNA表达均明显高于同时间点对照组(P<0.05),以第7天最高,第14天开始下降,与ELISA检测结果一致;第7,14天Col Ⅰ mRNA表达较弱,第21,28天表达增强,均高于对照组(P<0.05),与CVF结果一致,且Col Ⅰ mRNA与CVF呈正相关。结论:VMC急性期即出现OPN表达增加,至恢复期仍高于对照组,提示OPN可能参与VMC急性期的炎症反应,并促进恢复期胶原表达。
Objective： To study the mechanism of osteopontin （OPN） in viral myocarditis by observing the expression of OPN and collagen I （Col I） in mice myocardium. Methods： The viral myocarditis models were achieved by infection with myocarditic coxsackievirus B3 （CVB3）. The myocardium of mice was stained by HE and Masson staining, and the pathological scores and the collagen volume fraction （CVF）of myocardium were tabulated. The expression of Col I mRNA was measured by RT-PCR. The expression of OPN was detected by RT-PCR and ELISA. Results： The histopathological examination revealed a prevalence of myocardial cell necrosis and obvious inflammation changes at the 7th day post-infection. Subsequently the inflammatorylesions were gradually absorbed. At the 28th day, the inflammatory cells had almost disappeared and obvious fibrosis occurred. The pathological scores and the expression of OPN mRNA were higher than those of the control group （P〈0.05）, and reached the highest level at the 7th day （P〈0.05）.From the 14th day3 these parameters decreased,reflected also in the ELISA results. At the 7th day and the 14th daft the Col I expression was similar to that of control. Col I expression at the 21th and 28th days was higher than those of the control （P〈0.05）, and correlated positively to the CVF results. Conclusion： The OPN mRNA expression increased in acute stage of VMC, and higher than that of the control group when in recovery stage, suggesting that OPN might be related to the inflammatory response in acute stage of, and promote the collagen synthesis of recovery stage.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2012年第3期271-277,共7页
Journal of Central South University ：Medical Science
基金
湖南省卫生厅科研基金(B2008-019)
湖南省自然科学基金(09JJ5025)~~
