摘要
目的探讨孕妇血浆中游离胎儿DNA在非创伤性产前诊断中的可行性。方法用柱分离法提取46例孕妇血浆中胎儿DNA,用巢式聚合酶链反应(PCR)技术扩增其胎儿SRY基因,并引入内参照基因ATL1特异序列。结果 28例孕男胎的孕妇血浆中有26例经巢式PCR扩增出SRY基因,18例孕女胎的孕妇血浆中有17例经巢式PCR只扩增出ATL1基因。灵敏度和特异度分别为92.9%(26/28)和94.4%(17/18),总符合率为93.5%(43/46)。结论应用孕妇血浆中胎儿DNA作产前诊断灵敏度和特异度较高,是一种非创伤性产前诊断方法,具有广泛的临床应用前景。
Objective To investigate the feasibility of free fetal DNA from maternal plasma in non-invasive prenatal diagnosis.Methods Free fetal DNA was extracted by column seperation from 46 maternal plasma,and nested polymerase chain reaction（PCR） was used to amplify the SRY gene,and ATL1 gene specific sequence was amplified as an internal control.Results The SRY gene sequence was detected in 26 maternal plasmas from 28 pregnant women with male fetus.In 17 maternal plasmas from 18 pregnant women with female fetus,only the ATL1 gene sequence was detected.The sensitivity was 92.9%（26/28）,the specificity was 94.4%（17/18）,and the coincidence rate was 93.5%（43/46）.Conclusions Free fetal DNA from maternal plasma analysis is a non-invasive prenatal diagnosis and has the advantages of sensitivity and specificity,and it can improve in the clinical application.
出处
《检验医学》
CAS
2012年第3期159-162,共4页
Laboratory Medicine
关键词
胎儿DNA
巢式聚合酶链反应
产前诊断
Fetal DNA
Nested polymerase chain reaction
Prenatal diagnosis
