摘要
以水作为提取溶剂、银杏叶多糖提取率为指标,采用微波辅助提取法,在单因素试验的基础上,通过正交试验对银杏叶多糖的微波辅助提取工艺进行优化,并采用清除DPPH自由基、.OH和O2.模型对其体外抗氧化活性进行评价,并与VC进行比较。结果表明:微波辅助提取银杏叶多糖的最佳出工艺条件为微波功率480W、液料比30:1(mL/g)、提取时间8min、提取2次,多糖得率为14.70%。银杏叶多糖具有较强的清除DPPH自由基、.OH的能力,并与质量浓度呈一定正相关关系,清除O2.能力弱,清除率与多糖质量浓度的关系不显著。
Orthogonal array design method was applied to optimize the microwave-assisted water extraction of polysaccharides from Ginggo biloba leaves（GBLP） in order to maximize polysaccharide yield.Meanwhile,the antioxidant activity in vitro of polysaccharides from Ginggo biloba leaves was assessed by DPPH,hydroxyl and superoxide anion free radical scavenging assays and compared with vitamin C.The results showed that the optimal extraction conditions were microwave power of 480 W,material-to-liquid ratio of 1：30（g/mL）,and microwave treatment time of 8 min.Under these conditions,the extraction yield of polysaccharides was up to 14.70%.The extract obtained had a powerful scavenging activity against DPPH and hydroxyl free radicals in a concentration-dependent manner but a weak superoxide anion free radical scavenging activity with no significant concentration-dependent relationship.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第6期24-28,共5页
Food Science
关键词
微波辅助提取
银杏叶多糖
体外抗氧化活性
microwave-assisted extraction
polysaccharides from Gingko biloba leaves
antioxidant activity
