摘要
为制备抗鸡传染性喉气管炎病毒(ILTV)糖蛋白gD的单克隆抗体(MAb),本研究通过原核表达gD重组蛋白,纯化后免疫6周龄雌性BALB/c小鼠,经细胞融合筛选获得一株稳定分泌抗ILTV gD蛋白的杂交瘤细胞株,MAb亚型经鉴定为IgG1,轻链为κ链。Western blot结果显示,这株杂交瘤细胞分泌的MAb能够识别ILTV。ILTV gD蛋白的MAb的制备,为ILTV检测方法的建立奠定了基础。
To prepare monoclonal antibody （MAb） against glycoprotein D of infectious laryngotracheitis virus （ILTV）, the SP2/0 cells were fused with spleen cells of BALB/c mice immunized with the purified recombinant gD protein, a hybridoma cell line stably secreting antibody was obtained by scanning with indirect ELISA coating with purified ILTV as coating antigen. The MAb was identified to be IgG1 subclass with K light chain. Western blot analysis showed that MAb reacted positively with ILTV. The MAb developed could serve as a tool for further study on ILTV and development of detection method for ILTV infection.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2012年第3期232-234,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
兽医生物技术国家重点实验室基本科研业务费(SKLVBP201022)
中央级公益性科研院所基本科研业务费项目(ZGKJ201003)
黑龙江省杰出青年科学基金项目(JC200812)
关键词
传染性喉气管炎病毒
gD蛋白
原核表达
单克隆抗体
infectious laryngotracheitis virus
gD protein
prokaryotic expression
monoclonal antibody
