摘要
目的:观察活血药合用安神药对急性心肌梗死(AMI)大鼠心肌损伤的保护作用。方法:Wistar大鼠行冠状动脉左前降支结扎复制急性心肌梗死模型,分为假手术组、模型组、活血药组和活血药合用安神药组。给药组于造模当天连续给药至术后21 d,观察心脏射血分数(EF)和左心室缩短分数(FS)、左室舒张期内径(LVIDd)和左室收缩期内径(LVIDs)、心脏整体形态、HE染色及电镜观察等心功能和组织形态学变化。结果:和假手术组比较,模型组EF和FS显著下降(P<0.001),LVIDd,LVIDs明显增大(P<0.001),心肌梗死面积达左心室前壁的70%～90%,心肌纤维化、炎细胞浸润、肌纤维断裂和心肌细胞线粒体空泡样变等病理改变严重;和模型组比较,活血药和活血药合用安神药组EF和FS显著升高(P<0.05或P<0.01),LVIDd,LVIDs明显减小(P<0.05或P<0.01),心肌梗死面积约占左心室前壁的40%～60%,心肌纤维化、炎细胞浸润、肌纤维断裂和心肌细胞线粒体空泡样变等病理改变明显减轻;和活血药组比较,活血药合用安神药组EF和FS进一步升高,LVIDd,LVIDs进一步减小(P<0.05),心肌纤维化、炎细胞浸润、肌纤维断裂和心肌细胞线粒体空泡样变等病理改变进一步减轻。结论:活血药基础上合用安神药能进一步改善梗死后缺血心肌的结构和功能,有显著的增效作用,该作用可能与安神药的抗脂质过氧化,稳定心肌细胞膜和心肌线粒体膜,减轻心肌细胞损伤有关。
Objective： To observe the protective effect of combined administration of blood-activating drug and sedative drug on myocardial injury of acute myocardial infarction（AMI） rats.Method： The acute myocardial infarction（AMI） model was established by occluding the left descending coronary artery of Wistar rats.These rats were further divided into four groups（n=15 per group）： the sham-operated group,the AMI model group,the blood-activating drug group and the combined administration group.Result： Compared with the sham-operated group,the AMI model group showed significant decrease in ejection fraction（EF） and fractional shortening（FS）（P0.001） and notable increase in the left ventricular end-diastolic internal diameter（LVIDd） and left ventricular end-systolic internal diameter（LVIDs）（P0.01）,with the infarct area of left ventricular front wall up to about 70%-90%.Besides,tissue was severely replaced by collagen deposition and fibrosis,the sarcomeres disorganized and mitochondrial abnormalized.Compared with the AMI model group,the blood-activating drug group and the combined administration group showed significant increase in the values of EF and FS（P0.05 or P0.01） and obvious reduction in LVIDd and LVIDs（P0.05 or P0.01）,with the infarction area of left ventricular front wall up to about 40%-60%.The collagen deposition and myocardium fibrosis,the disorganized sarcomeres and mitochondrial abnormalities relieved significantly.And compared with blood-activating drug group,the combined administration group demonstrated further increase in the values of EF and FS and further decrease in LVIDd and LVIDs（P0.05）,the collagen deposition and myocardium fibrosis,the disorganized sarcomeres and mitochondrial abnormalities relieved even more in Huoxue plus Anshen prescription group.Conclusion： The combined administration of blood-activating drug and sedative drug can further improve cardiac structure and function after myocardial ischemia infarction and have an obvious synergistic effect which may be related to sedative drug＇s effect of resisting lipid peroxide,stabling myocardial cell membrane and mitochondrial membrane and relieving cardiac cell injury.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第7期1012-1016,共5页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09103-355)
关键词
活血药
安神药
急性心肌梗死
心肌损伤
blood-activating drug
sedative drug
acute myocardial infarction
myocardial injury
