摘要
目的:探讨七叶皂苷钠对一氧化碳中毒迟发性脑病大鼠模型细胞凋亡及Caspase-3表达的影响。方法:将30只大鼠随机分为正常对照组、一氧化碳中毒迟发性脑病大鼠模型对照组和七叶皂苷钠治疗组,原位末端标记法(TUNEL)检测细胞凋亡,免疫组化法检测Caspase-3的表达。结果:治疗组大鼠脑细胞凋亡率及Caspase-3表达较迟发性脑病对照组显著降低(P<0.01)。结论:七叶皂苷钠对一氧化碳中毒迟发性脑病的脑保护作用机制可能与干预脑细胞凋亡相关基因表达并减少神经细胞凋亡有关。
Objective： To observe the effects of Sodium Aescinate on cell apoptosis and the expression of caspase-3 in delayed encephalopathy rat model of carbon monoxide poisoning. Methods： Thirty rats were randomly divided into three groups, that is, normal group, delayed encephalopathy rate model of carbon monoxide poisoning control group and Sodium Aescinate treatment group. The cell apoptosis was detected by TUNEL stacning. The expression of caspase-3 was determined by immunohistochemical staining. Results： The cell apoptosis rate and the positive cell number ofcaspase-3 were significantly lower （P〈0.01） in Sodium Aescinate treatment group than those in the delayed encephalopathy after carbon monoxide poisoning control group. Conclusion： The protective effect of Sodium Aescinate on the cerebral tissue after carbon monoxide poisoning may be related with its intervening in the expression of apoptosis-related gene and decreasing the degree of nerve cell apoptosis.
出处
《现代生物医学进展》
CAS
2012年第5期865-867,共3页
Progress in Modern Biomedicine
