摘要
目的观察前列腺癌（PCa）患者外周血单个核细胞CD4^＋CD25^＋Foxp3^＋调节性T细胞的变化及其与国际肿瘤淋巴结转移（TNM）分期的关系。方法采用流式细胞术检测20例体检健康者（对照组）及43例PCa患者（患者组，临床TNM分期I期7例、II期18例、III期10例、IV期8例）外周血单个核细胞CD4^＋CD25^＋Foxp3^＋调节性T细胞数目，并计算CD4^＋CD25^＋Foxp3^＋调节性T细胞占CD4^＋T淋巴细胞的百分比：分析CD4^＋CD25^＋Foxp3^＋调节性T细胞与肿瘤分期的相关性。结果与正常对照组比，患者组外周血单核细胞CD4^＋CD25^＋Foxp3^＋Treg占CD4^＋T淋巴细胞的百分比及CD4^＋CD25^＋Foxp3^＋Treg绝对数均显著升高（P〈0．01，P〈0．05）。患者外周血单核细胞CD4^＋CD25^＋Foxp3^＋Treg占CD4＋T淋巴细胞的百分比及CD4^＋CD25^＋Foxp3^＋Treg绝对数均随TNM分期及病理分级的递增而逐步升高，其中Ⅳ期较其他各期显著升高，差异具统计学意义（P〈0．01，P〈0．05）；II期与III期患者PBMCCD4^＋CD25^＋Foxp3^＋Treg占CD4’T淋巴细胞的百分比及CD4^＋CD25^＋Foxp3^＋Treg绝对数均较I期明显升高，但II期与III期比无统计学意义（P〉O．05）；PCa高危组较中危组及低危组显著升高（P〈0．01，P〈0．05）。CD4^＋CD25^＋Foxp3^＋Treg／CD4^＋T及CD4^＋CD25^＋Foxp3^＋Treg绝对数均与TNM分期呈明显正相关（r分别为0．732、0．596，P〈0．01），与病理分级亦呈明显正相关（r分别为0．622、0．587，P〈0．01）。结论PCa患者外周血CD4^＋CD25^＋Foxp3^＋Treg数量及比例升高，其升高的程度与临床分期显著相关，监测PCa患者外周血CD4^＋CD25^＋Foxp3^＋Treg的水平可能有助于判断患者病情变化。
Objective To investigate the relationship between the changes of CD4^＋CD25^＋Foxp3^＋ regulatory T cells of peripheral blood in prostate cancer patients and the international staging of tumor. Methods The number of CD4^＋CD25^＋Foxp3^＋ regulatory T cell in peripheral blood of 43 patients with prostate cancer （staging of tumor： II18, III10 and 1V 8） and 20 healthy examiners were detected by the flow cytometry. The relationship between the changes of CD4^＋CD25^＋Foxp3^＋ regulatory T cells and the staging of tumor was statistically analyzed. Results Compared with that of the healthy control group, the proportion and number of CD4^＋CD25^＋Foxp3^＋ regulatory T cells of peripheral blood in prostate cancer patients were all increased significantly （P〈0.01, P〈0.05） alongside the gradual increase of the staging of tumor and risk level. The proportion and number of CD4^＋CD25^＋Foxp3^＋ regulatory T cell in patients of Stage IV were increased significantly than that in patients of other stages （P〈0.01, P〈0.05）. That in patients of Stage II and III was increased significantly than that of Stage I, and there was no statistical difference between Stage II and StageIII. （P〉0.05）. The proportion and number of CD4^＋CD25^＋Foxp3^＋ regulatory T cells in the high-risk group were increased significantly than that in the low-risk group and average-risk group （P〈0.01, P〈0.05）. The proportion and number of CD4^＋CD25^＋Foxp3^＋ regulatory T cells were positively correlated with the staging of tumor and risk level. Conclusion Higher proportion and number of CD4^＋CD25^＋Foxp3^＋ regulatory T cells are found in peripheral blood in prostate cancer patients , and they increase with the staging. The level of CD4^＋CD25^＋Foxp3^＋ regulatory T cells may be useful in evaluation of the patient＇s condition.
出处
《中国男科学杂志》
CAS
CSCD
北大核心
2012年第2期15-18,共4页
Chinese Journal of Andrology
关键词
前列腺肿瘤
肿瘤分期
T淋巴细胞
调节性
prostatic neoplasms
neoplasms staging
T-Lymphocytes
regulatory
