摘要
以红香椿为原料,采用有机溶剂浸提法,研究了浸提溶剂种类、浸提溶剂配比、浸提温度、浸提时间和料液比对红香椿叶绿素提取效果的影响;采用正交试验设计优化了红香椿叶绿素的提取工艺,并探讨了红香椿叶绿素的稳定性。结果表明,丙酮-无水乙醇混合液（1∶1）按料液比为1∶8在60℃浸提20h,红香椿叶绿素的提取效果最好,叶绿素得率可达684.4080μg/g;红香椿叶绿素具有很强的耐热性,在pH值为6~10的范围内稳定性,对葡萄糖稳定,但对柠檬酸不稳定;在自然光下易降解;Na＋、K＋、Ca2＋、Mg2＋、Fe3＋、Zn2＋等金属离子对红香椿叶绿素的稳定性有显著影响。
Using the leaf of red Toona Sinensis Roem as raw material,the extracting technology and the stability of red Toona Sinensis Roem through organic solvent extraction and orthogonal experiment were discussed in this paper.Influences of extraction solvents,solvent concentration,extraction temperature,extraction time and the ration of liquid to solid on extraction efficiency were studied.The result indicated that the extraction rate of chlorophyll reached 684.4080μg/g when extracted with the combination of acetone and anhydrous ethanol by same equal volume,at 60℃ for 20 h and 1：8 of liquid to solid ratio.The chlorophyll of red Toona Sinensis Roem had good tolerance for heat treatment,and stable when pH value in the range of 6~10.The chlorophyll were little affected by glucose but significantly affected by citric acid.It is easily degraded under the illumination condition.Metal ions including Na＋,K＋,Ca2＋,Mg2＋,Fe3＋,Zn2＋ significantly effect on the chlorophyll stability of red Toona Sinensis Roem.
出处
《中国酿造》
CAS
2012年第3期82-86,共5页
China Brewing
基金
山东省教育厅科技计划项目(J09LC69)
济宁市科技局优秀中青年科研创新资金项目(2010JNKJ104)
关键词
红香椿
叶绿素
有机溶剂
提取工艺
稳定性
red Toona sinensis Roem
chlorophyll
organic solvent
extraction technology
stability
