摘要
系统考察了55种非离子型化合物(包括11个氯代苯类、14个烷基苯类、22个多苯和多环芳烃类化合物、8个农药)在土壤淋溶柱色谱和较宽的淋洗剂(甲醇水)组成范围内容量因子(k′)与甲醇体积分数(φ)的关系。用自制加压装柱机将标准土壤干法装填成10mmi.d.×100mm的液相色谱柱,φ=0～0.80的等度淋洗剂的流速1mLmin-1,柱温(25.0±0.1)℃,用UV检测器在线检测。实验表明,logk′=logk′w+aφ+bφ2和logk′=logk′w-Sφ两式均可以用来描述保留规律,但后者因其计算简单、实验数据点不需太多,可更好地满足实验要求。还解释了同系列的同系物(甲基苯类、正构烷基苯类)和弱极性氯代苯类中存在的logk′,logk′w,S的碳(或氯)数规律,该规律中的斜率m和截距b间也存在线性相关,且均随φ的增大而线性地减少。
The relationship between capacity factors (k′) of 55 nonionic compounds and broad methanol volume percentage (φ) of methanol water eluent in soil leaching column chromatography (SLCC) was systematically investigated. The compounds consist of 11 chlorobenzenes, 14 alkylbenzenes, 22 polyphenyls and polycyclic aromatic hydrocarbons, and 8 pesticides. Reference soil was dry packed into a stainless steel chromatographic column (10 mm i.d.×100 mm) by a homemade pressurizing device, and isocratic methanol water mixture with φ from 0.0 to 0.80 eluated through the column at a flow rate of 1 mL·min -1 . The column was thermostated at (25.0±0.1) ℃, and chromatographic peak was monitored by an online ultraviolet detector. The results show that both equations, log k′ = log k ′ w+aφ+bφ 2 (1) and log k′= log k ′ w-Sφ (2), well fit the retention values. Equation (2) can be used practically due to few experimental data needed and simpler in formula. Explanation is also given for the existence of the carbon (or chlorine ) number rule for two classes of homologous series (i.e. methylbenzenes, n alkylbenzenes) and weak polar chlorobenzenes in the SLCC process. The slope and intercept of the rule are also well correlated, and both decreases linearly with increasing eluent φ value.
出处
《色谱》
CAS
CSCD
北大核心
2000年第1期5-9,共5页
Chinese Journal of Chromatography
关键词
土壤淋溶柱色谱
保留方程
有机调节剂
甲醇
soil leaching column chromatography (SLCC)
retention equation
organic modifier
methanol
carbon (or chlorine) number rule
