摘要
高效离子交换色谱法分离和纯化蛋白质时，蛋白质在色谱柱上的不可逆吸附使柱效降低，柱压升高。本文使用２％的胰蛋白酶溶液在３７℃下处理ＴＳＤｇａｌ ＣＭ－５ＰＷ和ＴＳＫｇｅｌＤＥＡＥ－５ＰＷ（７５×７．５ｍｍＩ．Ｄ．）离子交换色谱柱２４h后，柱压显著降低，柱效提高４～１３倍，交换容量也得到恢复。离子交换色谱术的恢复程度和交换容量与处理液的用量有关。
Irreversible adsorption of proteins on high-performance ion-exchange packings can result in column deterioration and damage. TSKgel CM-5PW column and a DEAN-5PW (75 × 7. 5 mm I. D. ) column fouled by irrevesible adsorption of proteins were treated with 2% trypsin solution under 37℃ for 24 hours, the back pressure was significantly declined, the number of theoretical Plate was increased by 4 to 13 times and ion-exchange capacity was restored. The extent of column regeneration was related with the volume and concentration of the trypsin solution.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第1期46-49,共4页
Chinese Journal of Analytical Chemistry
关键词
蛋白质
分离
纯化
离子交换柱
色谱分离
Protein, separation and purification, ion-exchang column, regeneration
