摘要
目的:观察C-erbB2基因shRNA表达质粒对结肠癌HT-29细胞生长抑制、细胞周期和凋亡的影响。方法:用MTT法及流式细胞仪分别检测pGenesil-erbB2实验组(PEG)、转染试剂对照组(TRCG)、阴性质粒对照组(NPCG)细胞对结肠癌细胞生长曲线、细胞周期及细胞凋亡率的影响。结果:pGenesil-erbB2实验组、转染试剂对照组、阴性质粒对照组的生长抑制率分别为39.65%、7.23%、8.05%,实验组明显高于其他两组(P<0.01);pGenesil-erbB2实验组、转染试剂对照组、阴性质粒对照组的G0/G1期细胞分别占74.93%、67.19%、68.05%,实验组明显高于其他两组(P<0.05);S期细胞分别占7.81%、14.02%、13.70%,实验组明显低于其他两组(P<0.05);pGenesil-erbB2实验组、转染试剂对照组、阴性质粒对照组的凋亡率分别为19.21%、3.13%、4.08%,实验组明显高于其他两组(P<0.01)。结论:C-erbB2基因siRNA重组质粒可以明显抑制结肠癌细胞的生长;可以将细胞周期阻滞于G0/G1期;可以明显增加结肠癌细胞的发生凋亡;说明C-erbB2基因在结肠癌的发生和发展中也起到非常重要的作用。
AIM： To study the changes of the cell growth, cell cycle distribution and cell apoptosis of colon cancer cell, HT-29, when C-erbB2 gene was knockdown by shRNA against C-erbB2. METHODS： Cell growth, cell cycle distribution and cell apoptosis were compared among three groups including plasmid experimental group （ PEG）, transfected reagent control group （TRCG）and negative plasmid control group （NPCG）. Cell growth was measured by MTT assay. Cell cycle distribution and cell apoptosis were detected by flow cytometry. RESULTS. The inhibition rate of cell growth of PEG, TRCG and NPCG were 39.65%, 7.23% and 8.05% respectively. The cell growth was significantly inhibited in PEG （ P〈0.01 ）. The cells of G0/G1 phase were 74. 93%, 67. 19%, 68. 0.5% respectively in PEG, TRCG and NPCG. The cells of G0/G1 phase in PEG were significantly more than those in TRCG and NPCG （P〈0.05）. While the cells of S phase were 7.81%, 14.02%, 13.70% in PEG, TRCG and NPCG respectively. The cells of S phase in PEG were significantly less than those in TRCG or NPCG （ P〈0.05 ）. The cell apoptosis rate were 19.21%, 3. 13%, 4.08% in PEG, TRCG and NPCG respectively. The cell apoptosis rate in PEG was significantly higher than those in TRCG or NPCG（P〈0.01）. CONCLUSION： Cell growth was inhibited by shRNA against C-erbB2 gene. Cell cycle was blocked in G0/G1 phase and apoptosis was induced by C-erbB2 shRNA. This indicates C-erbB2 gene plays important roles in the carcinogenesis and development of colon cancer.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2012年第4期371-373,共3页
Chinese Journal of Cellular and Molecular Immunology
