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rBTI蛋白对人肝癌HepG2细胞增殖、迁移及ROS产生的影响 被引量：3

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摘要 目的:研究重组荞麦胰蛋白酶抑制剂(rBTI)对HepG2细胞增殖、凋亡、迁移及胞内活性氧(ROS)的影响。方法:以不同浓度的rBTI蛋白(1.25 10μmol/L)作用肝癌HepG2细胞,通过MTT比色法检测rBTI蛋白对HepG2细胞增殖抑制作用;DAPI荧光染色法观察凋亡细胞的发生及形态学变化,流式细胞术(FCM)检测细胞内活性氧变化、划痕擦伤实验检测rBTI蛋白对HepG2细胞迁移的影响。结果:rBTI蛋白对HepG2细胞的生长具有明显的抑制作用,并呈剂量依赖效应,但对正常细胞HL-7702影响很小;DAPI染色发现细胞产生凋亡小体;细胞内活性氧水平检测结果表明rBTI可以诱导HepG2细胞内ROS水平显著升高;划痕擦伤实验显示rBTI在一定浓度范围内可以抑制HepG2细胞迁移。结论:rBTI蛋白能够诱导HepG2细胞发生凋亡并抑制细胞增殖和迁移。
作者 李玉英 贾海燕 郭慧 王转花
机构地区 山西大学生物技术研究所
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2012年第4期358-360,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目(30970611 31171659) 山西省自然科学基金资助项目(2011011035-4)
关键词 rBTI 肝癌 增殖 细胞迁移 活性氧
分类号 Q78 [生物学—分子生物学] R392.11 [医药卫生—免疫学]
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共引文献15

同被引文献32

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细胞与分子免疫学杂志
2012年 第4期

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