摘要
目的:筛选香附抗抑郁活性部位并初步探讨其抗抑郁作用机制。方法:小鼠ig香附各提取物给药量均为10 g.kg-1,阳性对照药氟西汀(0.08 g.kg-1),连续给药15 d后进行测试,采用小鼠强迫游泳实验和小鼠尾悬挂实验对香附醇提物及其各极性部位进行抗抑郁活性筛选,继而采用反相高效液相色谱法测定大脑额叶皮质中单胺类神经递质含量变化,探讨香附抗抑郁作用机制。结果:香附醇提取物的乙酸乙酯萃取部位和正丁醇萃取部位与对照品氟西汀类似,均能显著缩短小鼠游泳和悬尾的不动时间,乙酸乙酯萃取部位活性强于正丁醇萃取部位。乙酸乙酯萃取部位和正丁醇萃取部位可明显升高小鼠大脑额叶皮质5-羟色胺(5-HT)和多巴胺(DA)含量(P<0.01),去甲肾上腺素(NE)含量无明显改变。结论:香附醇提物乙酸乙酯萃取部位和正丁醇萃取部位对"行为绝望"动物模型有较明显的抗抑郁作用,其作用机制可能与调节脑内单胺类神经递质5-HT和DA的含量有关。
Objective：To screen the antidepressant fractions from Cyperus rotundus and investigate the possible a mechanism.Method： The extract and florentine were orally given to mice at a dosage of 10 g·kg-1 and 0.08 g·kg-1 per day,respectively.The swimming test and the tail suspension test were applied to observe the antidepressant effect of C.rotundus extracts.The content of serotonin（5-HT）,dopamine（DA） and noradrenalin（NE） was determined by RP-HPLC method to study the mechanism of C.rotundus extracts.Result： The ethyl acetate and n-butanol fractions from the ethanol extract of C.rotundus significantly shortened immobility time in swimming test and the tail suspension test.The ethyl acetate and n-butanol fractions from the ethanol extract of C.rotundus markedly increased the content of 5-HT and DA with no action of NE.Conclusion： The ethyl acetate and n-butanol fractions from the ethanol extract of C.rotundus can produce an antidepressant effect,the mechanism of which may be related to affecting the 5-HT and DA nervous system.They may be the active part of C.rotundus.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期191-193,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东湛江师范学院自然科学研究项目博士专项(ZL1009)
关键词
香附
抗抑郁作用
药理活性
作用机制
Cyperus rotundus
antidepressant effect
pharmacology activity
mechanism
