摘要
目的:建立HPLC测定明目地黄丸(浓缩丸)中马钱苷的含量。方法:采用Kromasil C18色谱柱(4.6 mm×250mm,5μm),流动相为[四氢呋喃-乙腈-甲醇(1∶8∶4)-0.05%磷酸溶液](8∶92),检测波长为236 nm,流速为1.0 mL.min-1,柱温为40℃,外标法计算含量。结果:马钱苷在40.43～1 213.02 ng有良好线性关系(r=1.000 0),平均回收率为98.2%,RSD0.4%(n=6)。结论:此方法前处理简便,准确可靠,可作为该制剂的质控方法之一。
Objective：To establish a RP-HPLC method to determine the content of loganin in Mingmu Shangqing pill.Method： The analysis was performed on a Kromasil C18 column（4.6 mm×250 mm,5 μm） with tetrahydrofuran-acetonitrile-methanol（1∶8∶4）-0.05% phosphoric acid（8∶92） as mobile phase at a flow rate of 1.0 mL·min-1,and at a column temperature of 40 ℃.The detection wavelength was at 236 nm.The content was calculated with external standard method.Result： The linear range of loganin was 40.43-1 213.02 ng（r=1.000 0）.The average recovery was 98.2%,and the RSD was 0.8%（n=6） Conclusion： This method was simple,accurate,reliable and suitable to be applied to the quality control for the preparation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期68-70,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十一五"科技支撑"重大新药创制"专项课题(2009ZX09308)
