摘要
目的:优选大孔吸附树脂分离纯化龙胆中龙胆苦苷、獐芽菜苦苷和獐芽菜苷的工艺条件。方法:采用高效液相色谱法对龙胆苦苷、獐芽菜苦苷和獐芽菜苷进行定量分析,通过考察静态和动态吸附、洗脱效果,筛选大孔吸附树脂分离纯化龙胆中环烯醚萜苷的最佳工艺条件。结果:最佳工艺为HPD300树脂,上样液质量浓度0.1 g.mL-1,吸附流速1.0 BV.h-1,上样量10 BV,吸附后的树脂柱先用2 BV蒸馏水洗脱,再用8 BV 30%乙醇以2 BV.h-1流速洗脱。结论:HPD300大孔树脂对龙胆中龙胆苦苷、獐芽菜苦苷和獐芽菜苷具有较好的分离纯化效果,优选工艺操作简单、稳定可行。
Objective：To optimize separation and purification technology conditions of gentiopicroside,swertia bitter glycoside and swertia glycoside from Gentiana scabra.Method： The content of gentiopicroside,swertia bitter glycoside and swertia glycoside was determined by HPLC,optimum separation and purification process conditions of iridoid glycoside from G.scabra was screened by static and dynamic adsorption,desorption tests.Result： Optimum technology was：HPD300 macroporous resin was selected,the concentration of sample solution was 0.1 g·mL-1,adsoption flow rate was 1.0 BV·h-1,dosage of sample liquid was 10 BV,eluted by 2 BV water,then eluted with 8 BV 30% ethanol at eution flow speed was 2.0 BV·h-1.Conclusion： Gentiopicroside,swertia bitter glycoside and swertia glycoside from G.scabra were purified effectively with HPD300 macroporous resin,optimized process was simple,stable and feasble.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期20-23,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
吉林省科技厅科研课题(200905113)
关键词
大孔吸附树脂
龙胆
龙胆苦苷
獐芽菜苦苷
獐芽菜苷
macroporous adsorption resin
Gentiana scabra
gentiopicroside
swertia bitter glycoside
swertia glycoside
