摘要
目的:观察低剂量伽玛刀照射实验性老龄癫痫大鼠海马神经元超微结构的变化。方法:采用青霉素定位浸润建立老龄大鼠癫痫动物模型,将57只老龄大鼠分为对照组、癫痫组和癫痫后伽玛刀照射组。照射周边剂量12Gy,等剂量曲线为50%,0.5 h～60 d后取靶区海马制备电镜样品,透射电镜观察。结果:对照组海马神经元的超微结构正常;癫痫大鼠可见神经元细胞器明显空化,部分神经元凋亡;伽玛刀照射组早期与癫痫模型组基本一致,中期和晚期部分结构得以恢复,线粒体修复较为明显。结论:老龄癫痫大鼠经低剂量伽玛刀照射,中、晚期海马神经元的超微结构改变得以修复,可为临床治疗老龄癫痫提供形态学依据。
Objective： To observe the ultrastructural changes in hippocampal neurons of epileptic aged rats after exposed to low-dose gamma knife radiation.Methods： The aged rat models of epilepsy were established by infiltration of penicillin into cortex.Fifty seven rats were divided randomly into control group,epilepsy model group and low-dose gamma knife radiation group.The radiation was carried out with a peripheral dose of 12 Gy and 50% isodose curve.The samples of hippocampus of the entral aged rats and experimental epileptic rats with and without exposure to gamma knife,irradiation were taken and for transmission electron microscopic observation.Results： In control aged rat,numerous lipofuscin granules appeared in the cytoplasm of apparentely normal hippocampal neurons.In epileptic rats the vacuolation of mitochondria and increase of lipofuscin granules in the cytoplasm of hippocampal neurons were obviously and apoptotic neurons were found in the late stage of epileptic rat.After low-dose gamma knife radiation,there was an obviously recovelry of normal ultrastrcture of the injured neuron at the late stage of treatment.Conclusion：Ultrastructural injury of hippocampal neurons of experimental epileptic aged rat was obviously recovery at the late stage of low dose gamma knife radiation treatment.
出处
《电子显微学报》
CAS
CSCD
2012年第1期47-50,共4页
Journal of Chinese Electron Microscopy Society
关键词
老龄大鼠
癫痫
伽玛刀
海马神经元
超微结构
aged rat
epilepsy
gamma knife
hippocampal neuron
ultrastructure
