摘要
目的探讨FISH法检测PML-RARa融合基因在急性早幼粒细胞白血病(APL)中的临床应用价值。方法对21例疑诊APL患者应用FISH技术检测PML-RARa融合基因。结果 21例疑诊患者中最后确诊为APL 15例,排除APL 6例。15例APL患者中PML-RARa融合基因阳性14例,PML-RARa融合基因阴性者1例,该患者经最后确诊为APL变异型,经诱导治疗后效果不佳。6例疑诊患者PML-RARa融合基因均为阴性,确诊为急性髓细胞白血病-M2。结论 FISH法检测PML-RARa融合基因敏感、可靠,对APL的确诊及指导治疗有重要价值。
Objective To investigate the application of fluorescence in situ hybridization （FISH） for the detection of PML-RARa fusion gene in the diagnosis and treatment of acute promyelocytic leukemia（APL）. Methods PML-RARa fusion gene detection was performed in 21 patients suspected of APL by FISH. Results Of the 21 suspected APL patients, fifteen cases were eonfirrned of APL. Positive PML-RARa fusion genes were found in 14 of the 15 APL patients,while one ease with negative PML-RARa fusion gene was confirmed to be variant APL and was treated with all-trans retinoie acid （ATRA）-induction therapy but the effieacy was poor. The remaining 6 suspected APL patients with negative PML-RARa fusion gene were eonfirmed to be acute myeloid leukemia-M2. Conclusion Detection of PML-RARa fusion gene by FISH is sensitive and reliable, and is of great value for the diagnosis and treatment of APL.
出处
《广西医学》
CAS
2012年第3期275-278,共4页
Guangxi Medical Journal
基金
广西医药卫生科研课题(桂卫Z2008057
桂卫Z2009133)
