摘要
目的 :研究霉酚酸酯 ,地塞米松对血管内皮细胞功能的影响 ,进一步探讨这两种免疫抑制剂抗炎作用的机制。 方法 :以脐静脉来源的内皮细胞为研究对象 ,通过建立体外内皮细胞血管发生 (angiogenesis)模型 ,观察并比较了两种免疫抑制剂对内皮细胞血管发生 ,内皮细胞迁移能力 (Scratchwoundclosureassay)和内皮细胞增生(3 H TdR掺入 )的影响。 结果 :霉酚酸酯对血管内皮细胞体外血管发生有着较强的抑制作用 ,而地塞米松的抑制作用相对较弱。此外 ,霉酚酸酯还能显著抑制血管内皮细胞增生和迁移能力 ,而地塞米松则对此无显著作用。结论 :在对血管内皮细胞功能的影响上 ,霉酚酸酯具有比地塞米松更为广泛和显著的抑制效应 ,这可能是霉酚酸酯对一些激素治疗无效的血管炎患者仍有良好疗效的分子机制之一。
To explore the effect of mycophenolate mofetil(MMF) and dexamethasone(DEX) on endothelial cells. METHODOLOGY A cell line of human umbilical vein endothelial cells (HUVEC) from ATCC was used in the study. In vitro angiogenesis assay model was established to verify the effect of MMF and DEX on the angiogenic activity in HUVEC. The migration ability of HUVEC was measured by the scratch wound closure assay and the cell proliferation was determined by 3H TdR incorporation assay. RESULTS MMF and DEX were found to inhibit angiogenesis of HUVEC in vitro, and this effect was more potent in MMF. The migration and proliferation ability of HUVEC was inhibited significantly by MMF, but not DEX. CONCLUSION As an immunosuppressant, MMF is not only effect on the lymphocytes, but also inhibit the angiogenesis, cell migration and proliferation of endothelial cells. These effects of MMF are quite different from dexamethason.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
2000年第1期48-51,共4页
Chinese Journal of Nephrology,Dialysis & Transplantation
关键词
血管内皮细胞
血管发生
霉酚酸酯
药理
vascular endothelial cells angiogenesis mycophenolate mofetil
