摘要
目的 :评价重组红细胞生成素 (rH EPO)对透析患者免疫功能的影响。 方法 :用流式细胞仪、双抗体夹心酶标法、ELISA法、MTT比色法以及淋巴细胞毒试验检测接受rH EPO治疗的慢性血透患者以及透析治疗不伴贫血患者的淋巴细胞亚群、细胞因子等免疫指标。 结果 :治疗组rH EPO治疗前淋巴细胞亚群、细胞因子与正常人、患者对照组比较有显著性差异 (P <0 0 5 ) ;治疗组rH EPO治疗前后CD3、细胞因子、淋巴细胞刺激指数(SI)、群体反应性抗体 (PRA)有显著变化 (P值分别为 <0 0 5和 <0 0 0 1)。 结论 :rH EPO不是直接影响患者免疫功能 ,而是增加RBC数量 ,间接通过红细胞表面LFA 3与淋巴细胞表面CD2受体直接结合 ,增加细胞因子的分泌 ,影响T 细胞功能。
To evaluate the effect and related mechanism of rh EPO on cellular immune functional parameters in hemodialysis patients. METHODOLOGY 30 rh EPO treated anemic patients on maintenance hemodialysis(treatment group),10 non anemic patients on maintenance hemodialysis(patient control group)and 10 healthy volunteers(control group)were involved in this study.T lymphocyte subgroups were determined with flow cytometry and monoclonal anti CD3,anti CD4 and anti CD8.Interleukine 2(IL 2),soluble IL 2 receptor and TNF α were determined with ELISA.Panel reactive antibody(PRA)and lymphocyte stimulation index(SI)were also determined. RESULTS Significant difference in pretreatment lymphocyte subsets were found among the treatment group,the patient control group and the control group.Levels of CD3,TNF α,IL 2 and lymphocyte stimulation index were significantly increased( P <0 05)and panel reactive antibody significantly decreased after treatment with rh EPO ( P <0 01). CONCLUSION rh EPO treatment in hemodialysis patients exerts its improving effect on immune function via the increment of circulating red blood cell count,which directly influences.T lymphocyte function through combination of RBC LFA 3 with lymphocyte CD2 to enhance cytokine secretion.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
2000年第1期39-43,共5页
Chinese Journal of Nephrology,Dialysis & Transplantation
