摘要
目的 :应用一氧化氮合酶 (NOS)竞争性抑制剂 L- Nω -硝基精氨酸甲酯 (L- NAME)减少面神经损伤后一氧化氮 (NO)的产生量 ,以探讨一氧化氮合酶抑制剂在面神经损伤后对面神经运动神经元的作用。方法 :选用豚鼠 4 0只 ,手术方法制作面神经离断的豚鼠模型 ,随机分为两组 ,在再生室内分别施加L- NAME和生理盐水 (SAL) ,运用光镜和透射电镜对面神经运动神经元 (FMNs)形态学的变化进行定性、定量观察。结果 :抑制剂组四周 FMNs存活率为 0 .70 4± 0 .0 34,而对照组四周 FMNs存活率为 0 .4 76± 0 .0 19;抑制剂组八周 FMNs存活率为 0 .782± 0 .0 2 6 ,而对照组八周 FMNs存活率为 0 .52 1± 0 .0 19。同时透射电镜下观察到不同程度的凋亡细胞。结论 :L- NAME对面神经运动神经元有保护作用 ,其机制可能与
Objective:To elucidate the role of Nitric Oxide Synthase (NOS) Antagonist,N Nitro L Anginine Methy1 Ester (L NAME) in the protection of facial motoneurons and regeneration of facial nerve following facial nerve transection.Method:The facial nerves of 40 guinea pigs were exposed and a segment of the nerve about 3 mm long was excided.The two ends of the transected nerve were sutured and enveloped in a silicone tubule to form a regeneration chamber.L NAME and saline were respectively injected into the regeneration chambers just after transecting.Their facial nucleus were identificated and stained with Cresy1 Violet.Facial motoneurons in right and left side were counted.Results:The study showed significantly greater motoneurons in the group of L NAME than that in the group of saline.By light microscope and transimission electron microscope,we observed apoptosis of facial motoneurons following facial nerve transection.Conclusions:L NAME might be good for the survival of facial motoneurons following facial nerve transection.The mechanism seemd to be related to the blockade of NO's neurotoxicity. [Chinese Arch Otolaryngol Head Neck Surg,2000;7(1):48~52 From the Department of Otolaryngology Head and Neck Surgery,Affiliated Renji Hospital of Shanghai Second Medical University,Shanghai,200001 (Dr.Feng Yunhai)]
出处
《耳鼻咽喉（头颈外科）》
2000年第1期48-52,共5页
Chinese Arch Otolaryngology-Head Neck Surg
