摘要
采用功率1 000 W的脉冲超声波处理黄粉虫幼虫水溶性蛋白,并对蛋白的结构变化进行研究.结果表明:在脉冲超声波处理时间25 min内,蛋白的紫外吸收随着处理时间的延长而增强;蛋白的表面疏水性在处理10 min时达到最大,随后逐渐降低;游离巯基的含量在脉冲超声波处理5min时稍有降低,随后逐渐增加;蛋白粒径随着处理时间的延长而减小,当处理时间达到20 min时,粒径显著增大;蛋白二级结构中α-螺旋的含量降低,β-链及无规则卷曲的含量升高;脉冲超声波处理后蛋白的变性聚丙烯酰胺凝胶电泳图谱未发生改变,表明蛋白的一级结构未受到影响.
The effects of pulse ultrasonic treatment with power of 1 000 W on water soluble protein con- formation of Tenebrio molitor （L.） larva were investigated. The results show that the ultraviolet absorp- tion of protein is increased within 25 rain ultrasonic treatment. The surface hydrophobicity has remarkable change with maximum value at l0 rain, and decreases gradually from l0 to 25 min. The free sulfhydryl （SH） content decreases slightly after pulse ultrasonic treatment for 5 min, and then increases gently. Pro- tein particle size decreases with treatment time from 0 to 15 rain, and increases significantly after 20 min treatment. The CD analysis indicates that a-helix content is decreased, while β-structure and random coil content are increased by pulse ultrasonic treatment. SDS-PAGE demonstrates that the primary structure of water soluble protein is not changed by pulse ultrasonic treatment.
出处
《江苏大学学报（自然科学版）》
EI
CAS
北大核心
2012年第2期130-133,186,共5页
Journal of Jiangsu University：Natural Science Edition
基金
国家"863"计划项目(2007AA10Z321)
关键词
脉冲
超声波
黄粉虫幼虫
水溶性蛋白
结构
pulse
ultrasonic
larva of Tenebrio molitor （L.）
water soluble protein
conformation
