摘要
目的 :探讨增殖细胞核抗原 (proliferating cell nuclear antigen,PCNA)在中耳胆脂瘤上皮中的表达和定位以及与中耳炎症的关系。方法 :采用 PCNA单克隆抗体对 16例中耳胆脂瘤组织和 6例正常外耳道皮肤 ,面部皮肤以及鼓膜进行免疫定位检测。结果 :PCNA阳性细胞仅见于正常外耳道、面部皮肤和鼓膜的基底细胞层和棘细胞层的开始部位 ,亦可见于胆脂瘤组织上皮全层。上皮下炎性细胞浸润愈重 ,PCNA阳性细胞率愈高。结论 :中耳胆脂瘤上皮组织具有过度增殖能力。
Objective:To determine the expression and localization of proliferating cell nucler antigen(PCNA)in human middle ear cholesteatoma,and to understand the effects on PCNA subepidermal inflammatory cell infiltration of cholesteatoma.Methods:The distribution of PCNA in 16 cholesteatoma tissues and 6 normal external ear canal skin or facial skin and tympanic membrane were studied immunohistochemically with paraffin embedded sections.Results:In all cholesteatoma tissue samples,a large number of PCNA positive cells were observed in all layers of epithelium,whereas in normal external ear canal skin or facial and tympanic membrance,PCNA demonstrated the pre sence of proliferating cells in the basal and subrabasal cell layers.The PCNA positive rate was higher when subepidermal inflammatory cell infiltration was more severe.Conclusions:This study showed that cholesteatoma epidermis was in the state of hyperproliferation up to superior layers.The subepidermal inflammation was a possible cause of the keratinocytes hyperproliferation. [Chinese Arch Otolaryngol Head Neck Surg,2000;7(1):37~40 From the Department of Otolaryngology,PLA 163 Hospital,Changsha,410003 (Dr.Li Zhengxian)]
出处
《耳鼻咽喉（头颈外科）》
2000年第1期37-40,共4页
Chinese Arch Otolaryngology-Head Neck Surg
