摘要
目的:研究胃复春散剂对肿瘤坏死因子(TNF-α)诱导人胃上皮GES-1细胞肠化的影响,进而探讨胃复春治疗慢性胃炎的分子机制。方法:体外培养人胃上皮细胞GES-1,实验分为对照组、模型组和药物预处理组,用RT-PCR法检测细胞表面肠黏膜标志物黏蛋白(MUC2)及胃黏膜标志物性别决定区Y框蛋白2(SOX2)mRNA的表达。结果:胃复春可下调细胞中MUC2mRNA的表达,上调(SOX2)mRNA的表达,且药物干预组与模型组相比均P<0.05。结论:胃复春可能通过下调MUC2和上调SOX2的表达来实现抑制肠化的过程。
Objective：exploring the effectiveness of Weifuchun on TNF-α that induces the intestinal metaplasia of GES-1 cells to discuss Weifuchun＇s possible mechanism of the treatment of chronic gastritis.Methods：cultivating human gastric epithelial cells GES-1 in vitro.And the experiment can be divided into control group,model group and drug pre-treatment group,using RT-PCR method to test the expression of intestinal mucosa MUC2 and gastric markers SOX2mRNA.Results：Weifuchun can decrease the expression of MUC2mRNA and increase the expression of SOX2mRNA,with P 0.05 by the comparison of the drug intervention group and model group.Conclusion：Weifuchun can implement the process of intestinal metaplasia inhibition by decreasing MUC2 expression and increasing SOX2 expression.Hence,it provides a new evidence for Chinese Traditional Medicine Weifuchun to prevent and treat the chronic gastritis.
出处
《内蒙古中医药》
2012年第6期39-41,共3页
Inner Mongolia Journal of Traditional Chinese Medicine
