摘要
背景:目前周围神经去细胞的方法较多,应用较多的是化学萃取法和液氮冻融法,但是化学萃取法耗时较长,而液氮冻融法则去除细胞不彻底。作者在查阅大量文献和多次实践的基础上,最终确定了冻融+化学萃取+机械振荡的优化法对坐骨神经进行脱细胞处理。目的:对优化法制备的去细胞坐骨神经天然支架进行形态学分析,并对其成分进行鉴定。方法:取Wistar大鼠双侧坐骨神经,随机分为2组,对照组不做特殊处理,实验组用冻融+化学萃取+机械振荡的优化法进行脱细胞处理。结果与结论:用优化法制备的去细胞坐骨神经天然支架呈三维管状立体结构,许旺细胞、髓鞘及轴突去除完全。经免疫组化鉴定,Ⅰ型、Ⅲ型胶原和层粘连蛋白等细胞外基质主要成分得到保留。
BACKGROUND： Currently, there are so many acellular methods used in the peripheral nerve, and two common methods are chemical extraction and frozen-thawing method. However, chemical extraction has the disadvantage of time-consuming and frozen-thawing method cannot achieve complete cell-free. Freeze-thaw＋chemical extraction＋mechanical vibration were chosen as optimization method for acellular processing of sciatic nerve in this study based on a large amount of literatures and repeated practice. OBJECTIVE： To analyze the morphology and composition of the natural acellular sciatic nerve scaffold prepared by optimization method. METHODS： Bilateral sciatic nerves from Wistar rats were randomly divided into two groups. The control group did not receive special treatment. The experimental group was treated with freeze-thaw＋chemical extraction＋mechanical vibration to prepare acellular scaffold. RESULTS AND CONCLUSION： Schwann cells, myelin sheaths and axons were removed completely from the optimized acellular sciatic nerve scaffold. And the scaffold showed a three-dimensional tubular structure. Extracellular matrixes of type Ⅰ collagen, type Ⅲ collagen and laminin were retained.
出处
《中国组织工程研究》
CAS
CSCD
2012年第8期1367-1370,共4页
Chinese Journal of Tissue Engineering Research
