摘要
[目的]建立测定生姜中6-姜酚的高效液相色谱（HPLC）法。[方法]以100倍80%的甲醇热回流提取生姜2 h后用HPLC法测定;采用依利特C18反相色谱柱（250 mm×4.6 mm,5μm）;以异丙醇-甲醇-冰乙酸-水（12∶38∶4∶36,V/V/V/V）为流动相;流速0.8 mL/min;紫外检测波长280 nm;柱温34℃。[结果]6-姜酚在0.021 6~0.432 0μg的质量范围内与峰面积线性关系良好（r2=0.999 2,n=6）,回收率为（100.79±2.84）%,RSD为2.84%。[结论]本法操作简便,灵敏度高,快速可靠,可用于生姜中6-姜酚含量的测定。
[Objective] To establish a HPLC method for the determination of 6-gingerol in ginger. [Methods] Ginger was extracted by heat reflux with 80% methanol for 2 hours, the drug ratio was 1：100, and the Hypersil C18 ODS （250 mm × 4.6 mm, 5 μm） column was used to determine. The mobile phase consisted of methanol-water-isopropylalcohol-glacial acetic acid （38：36：12：4）; The flow rate was 0.8 mL/min. The detection wavelength was 280 nm. The column temperature was kept at 34℃. [Results] The linear range was between 0.021 6-0.432 0 μg. And the average recovery of the method was （100.79±2.84） % and the RSD was 2.84%. [Conclusion] The results show that this assay method is simple and accurate. And the method can be used to determine the contents of 6-JF in Zingiber officinale Rose.
出处
《天津中医药大学学报》
CAS
2012年第1期36-38,共3页
Journal of Tianjin University of Traditional Chinese Medicine
基金
天津市发展计划项目(033180611)
