摘要
采用试剂γ-氯丙基三乙氧基硅烷(CPTES)对介孔硅材料SBA-15进行表面改性,并通过红外图谱(FT-IR)和N2吸附脱附等温图(BET)对其进行表征。结果表明:改性前原材料的比表面积为460.9 m2/g,改性后材料比表面积提高到512.0 m2/g。利用改性前和改性后的SBA-15对猪胰脂肪酶进行固载实验,并对实验结果进行比较,发现改性后的SBA-15在脂肪酶活性、pH环境适应性、热耐受性和可操作性都优于改性前的SBA-15,在最优条件下的酶活力提高超过60%。
Media for enzyme immobilization were prepared by T-chloropropyl triethoxysilane （CPTES） group. The prepared modifying mesoporous silica SBA-15 with support was characterized with FT-IR and BET method and exhibited by high specific surface area and uniform pore size. Furthermore, unmodified SBA-15 and the modified SBA-C1 materials were used for porcine pancreas lipase immobilization. The re- sults were compared with that of enzyme activity, activity retention and stability. The modified SBA-15 material with CPFES showed good performances for porcine pancreas lipase immobilization. Compared with unmodified SBA-15, the adsorption capacity of SBA-C1 to porcine pancreas lipase was improved more than 60%.
出处
《生物加工过程》
CAS
CSCD
2012年第2期40-44,共5页
Chinese Journal of Bioprocess Engineering
基金
国家重点基础研究发展计划(973计划)资助项目(2009CB72470)
关键词
酶
固定化
介孔
分子筛
SBA-15
enzyme
immobilization
mesoporous
molecular sieves
SBA-15
