摘要
基于分子系统学方面对蝗总科7科20种昆虫的系统发育进行了探讨.采用PCR产物直接测序法测定了该20种昆虫的核基因28SrDNA的部分片段序列,并从GenBank中下载的摩门螽蟖和变色乌蜢作为外群,采用最大简约法(MP)构建了分子系统树.在获得的504bp序列中,有340个保守位点,161个变异位点,45个简约信息位点;G+C约占69.8%,A+T约占30.2%.分子系统树表明:蝗总科7科中槌角蝗科、网翅蝗科和剑角蝗科的分类地位有待进一步的研究,而其他科的分类地位基本与形态学分类一致.
Partial species of Acridoidea （20 species, 7 families）were studied in molecularsystematics. The partial 28SrDNA gene sequence Anabrus simplex and Erianthus versicolor were relationships of these species from family level were （MP） method. There were 340 conserved sites, 161 sites, and G＋ C was about 69.8% and A ＋ T was only about 30.2 % in the 504 nucleotides of the data. The results showed that taxonomic status of some specie, of Gomphoceridae, Arcypteridae and Acrididae should be researched further, however, taxonomic status of some species of other 4 families are consistent with morphological taxonomy in Acridoidea.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第2期67-70,75,共5页
Journal of Shaanxi Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(39391800)
