期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

溶血磷脂酸对上皮细胞整合素β_6表达的影响 被引量：2

Effect of lysophosphatidic acid on expression of integrin β_6 in epithelial cells
暂未订购
导出
摘要 目的:探讨溶血磷脂酸(LPA)在体外对整合素β_6(ITGB6)表达的影响,并进一步研究LPA诱导的活化转化生长因子β(TGF-β)在此过程中的作用。方法:原代培养的正常人支气管上皮(NHBE)细胞接种于6孔板中,经LPA诱导,收集细胞,分别利用RT-PCR和流式细胞术检测ITGB6 mRNA及细胞表面蛋白的表达;利用转染有TGF-β应答性纤溶酶原激活物抑制剂1(PAI-1)启动子片段并融合萤火虫荧光报告基因片段的转化貂肺上皮细胞(TMLC)作为TGF-β活性报告细胞检测活化的TGF-β。结果:(1)10μmol/L LPA诱导2 h后,ITGB6mRNA在上皮细胞中表达显著增加,具有明显的时间依赖性。(2)10μmmol/L LPA诱导4 h后,上皮细胞表面ITGB6蛋白表达明显增加。(3)抗α_vβ_6抗体可阻断LPA诱导的活化TGF-β,但不能阻断LPA诱导的ITGB6 mRNA的表达。结论:(1)LPA可诱导上皮细胞ITGB6 mRNA和细胞表面蛋白的表达。(2)LPA诱导的ITGB6 mRNA表达不依赖LPA诱导的TGF-β活化。 AIM：To investigate the effect of lysophosphatidic acid（LPA） on the expression of integrin B_6（ITGB6） for determining the role of transforming growth factor B（TGF-B） activation induced by LPA in this process. METHODS：Normal human bronchial epithelial（NHBE） cells were primarily cultured in 6 well plate and stimulated with LPA.The mRNA expression of ITGB6 and the level of cell surface ITGB6 protein were detected by RT-PCR and flow cytometry, respectively.The activity of active TGF -βinduced by LPA was measured by the method of transformed mink lung epithelial cells（TMLC） transfected with TGF -βresponsive plasminogen activator inhibitor 1（PAI-1 ） promoter fused with firefly luciferase reporter gene.RESULTS：After stimulated with LPA at concentration of 10μmol/L for 2 h,the mRNA expression of ITGB6 in epithelial cells was significantly increased in a time-dependent manner.The results of flow cytometry showed that the protein level of ITGB6 on cell surface was obviously increased after treated with LPA at concentration of 10μmol/L for 4 h.The active TGF -βinduced by LPA in epithelial cells was blocked by anαvB6 blocking antibody. However,α_vP_6 blocking antibody failed to inhibit the mRNA expression of ITGB6 induced by LPA.CONCLUSION： LPA induces the mRNA and cell surface protein of ITGB6 in epithelial cells.The up-regulated ITGB6 expression by LPA is independent on LPA-induced TGF -βactivation.
作者 许铭炎 邓小玲 陈锡和 杨利和 姚小武 傅玉才
机构地区 汕头大学医学院第二附属医院口腔科 汕头大学医学院细胞生物与遗传学教研室 汕头大学医学院细胞衰老实验室
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2012年第3期488-491,共4页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No.81072208) 广东省自然科学基金资助项目(No.S2011010005106) 广东省医学科研基金资助项目(No.A2010407) 汕头大学医学院基础与临床科研基金资助项目
关键词 溶血磷脂酸 整合素β6 上皮细胞 转化生长因子Β Lysophosphatidic acid Integrinβ6 Epithelial cells Transforming growth factor beta
分类号 R735.35 [医药卫生—肿瘤]
  • 相关文献

参考文献12

  • 1Breuss JM,Gillett N,Lu L,et al.Restricted distribution of integrin beta 6 mRNA in primate epithelial tissues[J]. J Histochem Cytochem,1993,41(10):1521-1527.
  • 2Huang XZ,Wu JF,Cass D,et al.Inactivation of the integrin beta 6 subunit gene reveals a role of epithelial integrins in regulating inflammation in the lung and skin[J].J Cell Biol,1996,133(4):921-928.
  • 3Huang X,Wu J,Spong S,et al.The integrinαvβ6 is critical for keratinocyte migration on both its known ligand, fibronectin,and on vitronectin[J].J Cell Sri,1998,111 (Pt 15):2189-2195.
  • 4Niu J,Gu X,Ahmed N,et al.Theαvβ6 integrin regulates its own expression with cell crowding:implications for tumour progression[J].Int J Cancer,2001.92(1 ): 40-48.
  • 5张琦,胡燕燕,彭程,牛卫博,刘恩宇,贺兆斌,赵传宗,牛军.αvβ6-ERK直接通路参与去甲斑蝥素诱导HT-29结肠癌细胞凋亡[J].中国病理生理杂志,2011,27(8):1525-1530. 被引量：3
  • 6Thomas GJ,Nystrom ML,Marshall JF.αvβ6 integrin in wound healing and cancer of the oral cavity[J].J Oral Pathol Med,2006,35(1 ):1-10.
  • 7Sheppard D,Cohen DS,Wang A,et al.Transforming growth factor beta differentially regulates expression of integrin subunits in guinea pig airway epithelial cells[J].J Biol Chem,1992,267(24):17409-17414.
  • 8周志斌,张志珺.溶血磷脂酸与血管平滑肌细胞表型转化[J].中国病理生理杂志,2009,25(6):1228-1231. 被引量：4
  • 9Lin ME,Herr DR,Chun J.Lysophosphatidic acid(LPA ) receptors:signaling properties and disease relevance[J]. Prostaglandins Other Lipid Medial,2010,91(3-4):130-138.
  • 10Xu MY,Porte J,Knox AJ,et al.Lysophosphatidic acid inducesαvβ6 integrin-mediated TGF-βactivation via the LPA2 receptor and the small G protein Gα_q[J].Am J Pathol,2009,174(4):1264-1279.

二级参考文献38

  • 1Wei-weiAN,Xian-fengGONG,Min-weiWANG,Shin-ichiTASHIRO,SatoshiONODERA,TakashiIKEJIMA.Norcantharidin induces apoptosis in HeLa cells through caspase, MAPK, and mitochondrial pathways[J].Acta Pharmacologica Sinica,2004,25(11):1502-1508. 被引量：15
  • 2Clària J.Regulation of cell proliferation and apoptosis by bioactive lipid mediators[J].Recent Patents Anticancer Drug Discov,2006,1(3):369-382.
  • 3Tigyi G.Physiological responses to lysophosphatidic acid and related glycerol-phospholipids[J].Prostaglandins,2001,64(1-4):47-62.
  • 4Seewald S,Sachinidis A,Dusing R,et al.Lysophosphatidic acid and intracellular signalling in vascular smooth muscle cells[J].Atherosclerosis,1997,130(1-2):121-131.
  • 5Kim J,Keys JR,Eckhart AD.Vascular smooth muscle migration and proliferation in response to lysophosphatidic acid (LPA) is mediated by LPA receptors coupling to Gq[J].Cell Signal,2006,18(10):1695-1701.
  • 6Xu YJ,Aziz OA,Bhugra P,et al.Potential role of lysophosphatidic acid in hypertension and atherosclerosis[J].Can J Cardiol,2003,19(13):1525-1536.
  • 7Siess W,Tigyi G.Thrombogenic and atherogenic activities of lysophosphatidic acid[J].J Cell Biochem,2004,92(6):1086-1094.
  • 8Yu HX,Terra CG,Kathryn EM,et al.Lysophosphatidic acid as an autocrine and paracrine mediator[J].Biochim biophys Acta,2002,158(2):270-281.
  • 9Tigyi G,Parrill AL.Molecular mechanisms of lysophosphatidie acid action[J].Prog Lipid Res,2003,42(6):498-526.
  • 10Chen X,Yang XY,Wang ND,et al.Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction[J].Scand J Clin Lab Invest,2003,63(7-8):497-503.

共引文献10

同被引文献22

  • 1黄培堂,王嘉玺,朱厚照,等(译).2002.分子克隆实验指南[M].第3版.北京:科学出版社:224-233
  • 2Bandyopadhyay A,Raghavan S.Defining the role of integrin αvβ6 in cancer[J] .Curr Drug Targets,2009,10(7):645-652.
  • 3Li HX,Zheng JH,Fan HX,et al.Expression of αvβ6 integrin and collagen fibre in oral squamous cell carcinoma:association with clinical outcomes and prognostic implications[J] .J Oral Pathol Med,2013,42(7):547-556.
  • 4Bates RC,Bellovin DI,Brown C,et al.Transcriptional activation of integrin β6 during the epithelial-mesenchymal transition defines a novel prognostic indicator of aggressive colon carcinoma[J] .J Clin Invest,2005,115(2):339-347.
  • 5Azare J,Leslie K,Al-Ahmadie H,et al.Constitutively activated Stat3 induces tumorigenesis and enhances cell motility of prostate epithelial cells through integrin β6[J] .Mol Cell Biol,2007,27(12):4444-4453.
  • 6Sullivan BP,Kassel KM,Manley S,et al.Regulation of transforming growth factor-β1-dependent integrin β6 expression by p38 mitogen-activated protein kinase in bile duct epithelial cells[J] .J Pharmacol Exp Ther,2011,337(2):471-478.
  • 7Zhang Q,Stovall DB,Inoue K,et al.The oncogenic role of Yin Yang 1[J] .Crit Rev Oncog,2011,16(3-4):163-197.
  • 8Dahl JA,Collas P.A rapid micro chromatin immunoprecipitation assay (ChIP)[J] .Nat Protoc,2008,3(6):1032-1045.
  • 9Zygogianni AG,Kyrgias G,Karakitsos P,et al.Oral squamous cell cancer:early detection and the role of alcohol and smoking[J] .Head Neck Oncol,2011,3:2.
  • 10Pfau JD,Taylor RK.Mutations in toxR and toxS that separate transcriptional activation from DNA binding at the cholera toxin gene promoter[J] .J Bacteriol,1998,180(17):4724-4733.

引证文献2

二级引证文献1

中国病理生理杂志
2012年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部